CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells.
- Author:
Ji-Chen WU
1
;
Sai-Di JIN
2
;
Jia-Hang SONG
2
;
Xin-Qi LIU
2
;
Wen-Jun MA
1
;
Lin CHANG
1
;
Xiao-Xiang GUAN
2
;
Ming-Yu ZHANG
2
;
Jia-Qi LIU
1
;
Hui FU
1
;
Ying WANG
3
,
4
;
Chao-Qian XU
5
Author Information
1. College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China.
2. College of Pharmacy, Harbin Medical University, Harbin 150081, China.
3. College of Basic Medicine, Mudanjiang Medical University, Mudanjiang 157400, China. wangxingliang78@
4. com.
5. College of Pharmacy, Harbin Medical University, Harbin 150081, China. xuchaoqian@ems.hrbmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Humans;
Arsenic Trioxide/pharmacology*;
RNA, Circular;
bcl-2-Associated X Protein/metabolism*;
RNA, Small Interfering/metabolism*;
Apoptosis;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Human Umbilical Vein Endothelial Cells/metabolism*;
RNA, Messenger/metabolism*
- From:
Acta Physiologica Sinica
2022;74(5):763-772
- CountryChina
- Language:Chinese
-
Abstract:
The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As2O3). Human umbilical vein endothelial cells (HUVECs) were treated with 0-15 μmol/L As2O3 for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As2O3 was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As2O3 group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As2O3. Knockdown of circRNA-0028171 by siRNA promoted As2O3-induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As2O3.
