Leech extract inhibits VEGF expression in WERI-RB-1 cells possibly via VEGF/PI3K/AKT signal pathway
10.3980/j.issn.1672-5123.2022.12.04
- VernacularTitle:水蛭提取液通过VEGF/PI3K/AKT通路抑制WERI-RB-1细胞表达VEGF
- Author:
Yuan-Yuan LI
1
;
Yan-Lin ZHENG
1
;
Jian-Chao LI
1
;
Yan-Nian HUI
1
;
Shi-Hang MA
1
;
Hui HUANG
1
;
Fang WANG
1
;
Hong-Jie MA
1
Author Information
1. Department of Ophthalmology, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710000, Shaanxi Province,China
- Publication Type:Journal Article
- Keywords:
leech extract;
retinoblastoma;
vascular endothelial growth factor(VEGF);
phosphatidylinositol 3-kinase/protein kinae B(PI3K/AKT);
signal pathway
- From:
International Eye Science
2022;22(12):1955-1959
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of leech extract on the expression of vascular endothelial growth factor(VEGF)in retinoblastoma(WERI-RB-1 cells)and the related molecular mechanism.METHODS: WERI-RB-1 cells cultured in vitro were divided into control group, 0.04U/mL leech extract group and 0.08U/mL leech extract group. The control group was cultured with complete medium for 48h, and the leech extract group was cultured with 0.04 and 0.08U/mL drug-containing medium for 48h, respectively. The expression level of VEGF in conditioned medium of cell culture was detected by ELISA. The mRNA levels of hypoxia-inducible factor-1a(HIF-1a)and matrix metalloproteinase-9(MMP-9)were detected by RT-PCR. The expression levels of HIF-1a, MMP-9, phosphatidylinositol 3-kinase(PI3K)and human phosphorylated AKT(p-AKT)were detected by Western Blot.RESULTS: Compared with the control group, the expression of VEGF in the conditioned medium with the concentration of 0.04U/ mL and 0.08U/mL of leech extract was decreased(P<0.05), and the inhibition rate was 32.43% and 38.92%, respectively. The expression levels of HIF-1a and MMP-9 mRNA were significantly decreased(P<0.05). The inhibition rates of HIF-1a mRNA expression of the two leech extracts were 27.64% and 24.75%, respectively. The inhibition rate of MMP-9 mRNA expression was 43.97% and 51.48%, respectively. The protein expression levels of HIF-1a, MMP-9, PI3K and p-AKT were significantly decreased compared with the control group(P<0.05). The inhibition rates of 0.04 and 0.08U/mL leech extracts on the protein expression of HIF-1a, MMP-9, PI3K and p-AKT were 55.81% and 43.85%, 39.49% and 47.23%, 33.27% and 29.83%, 52.07% and 30.21%, respectively.CONCLUSION:Leech extract may inhibit the expression of VEGF in WERI-RB-1 cells via the VEGF/PI3K/AKT pathway or HIF-1a and MMP-9 factors.
