Application of Optical Microscopic Imaging Technology in Liquid-Liquid Separation Research
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0319
- VernacularTitle:光学显微成像技术在液-液相分离研究中的应用
- Author:
Yuan-jun GUAN
1
;
Xian-cai MA
2
Author Information
1. Core Facilities for Medical Science， Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080，China
2. Key Laboratory of Tropical Disease Control （Sun Yat-sen University），Ministry of Education，Guangzhou 510080，China
- Publication Type:Review
- Keywords:
liquid-liquid phase separation;
optical microscopic imaging;
confocal microscopy;
fluorescence recovery after photobleaching;
super resolution
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):504-510
- CountryChina
- Language:Chinese
-
Abstract:
In the past decade， scientists have found that liquid-liquid separation plays an important role in many physiological processes and plays many biological functions. Its abnormal change will lead to a variety of diseases. In the study of liquid-liquid separation in biology， optical microscopy is the main research tool. This paper describes the application and comparison of several important optical microscopic imaging technologies in the study of liquid-liquid phase separation， to explain the existence and formation process of phase separation， obtain the mobility and diffusion data. The characteristics of diffusion and material exchange detected by fluorescence recovery after photobleaching （FRAP） in confocal microscopy are described in detail， and the imaging conditions， precautions and parameters are summarized. That might provide the basis for scientific researchers to choose the technology.
