Effects of Chaihu xianxiong decoction containing serum on HUVEC injury induced by high-fat serum and its mechanism
- VernacularTitle:柴胡陷胸汤含药血清对高脂血清致人脐静脉内皮细胞损伤的影响及机制
- Author:
Jianxiang WANG
1
;
Yang LIAO
2
;
Qiong YI
1
;
Xinyu CHEN
3
Author Information
1. Dept. of Critical Care Medicine，the First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，China
2. School of Graduate，Hunan University of Chinese Medicine，Changsha 410208，China
3. Dept. of Cardiovascular Medicine，the First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，China
- Publication Type:Journal Article
- Keywords:
Chaihu xianxiong decoction;
containing serum;
high-fat serum;
endothelial cells;
inflammatory reaction;
Toll-like
- From:
China Pharmacy
2022;33(20):2493-2497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effects of Chaihu xianxiong decoction containing serum on human umbilical vein endothelial cells ECV 304 injury induced by high -fat serum and its mechanism . METHODS The high -fat serum and Chaihu xianxiong decoction containing serum were prepared . ECV304 injury was induced by high -fat serum ，and the intervention experiments were conducted with low -concentration，medium-concentration and high -concentration（3.47，6.94，13.88 g/kg，by crude drug ）of Chaihu xianxiong decoction containing serum . The proliferation rate ，the levels of related inflammatory factors [tumor necrosis factor -α（TNF-α），interleukin-6（IL-6）] and vascular endothelial function indexes [endothelial nitric oxide synthase （eNOS），nitric oxide （NO）and endothelin -1（ET-1）] were all detected in each group . mRNA expressions of intercellular adhesion molecule-1（ICAM-1）and transforming growth factor -β1（TGF-β1）were detected ，and the expression of Toll -like receptor 4 （TLR4），nuclear factor -κB inhibitor α（IκBα），phosphorylated I κBα（p-IκBα）protein and the ratio of phosphorylated nuclear factor-κB p 65（p-NF-κB p 65）to NF -κB p 65 were also detected . RESULTS Compared with control group ，the proliferation rate of cell，the levels of TNF -α，IL-6 and ET -1，mRNA expression of ICAM -1，protein expressions of TLR 4 and p -IκBα and p -NF-κB p65/NF-κB p 65 ratio were significantly increased in the model group （P＜0.05）；the levels of eNOS and NO ，mRNA expression of TGF-β1 and protein expression of I κBα were significantly decreased （P＜0.05）. Compared with model group ，above indexes were all reversed significantly in low -concentration，medium-concentration and high-concentration groups of Chaihu xianxiong decoction （P＜0.05）， while the Chaihu xianxiong decoction high concentration group were all better than low -concentration and medium-concentration groups （P＜0.05）. CONCLUSIONS Chaihu xianxiong decoction can improve endothelial cell injury induced by high -fat serum . Its mechanism of action may be associated with reducing the levels of inflammatory factors and inhibiting the activation of TLR 4/NF-κB signaling pathway .
