Application of 3D-printed bredigite scaffolds in Onlay graft in rats
10.12016/j.issn.2096-1456.2023.01.004
- Author:
ZHANG Zhen
1
;
LI Jiayang
2
Author Information
1. Department of Oral & Maxillofacial-Head & Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University & National Center for Stomatology & National Clinical Research Center for Oral Diseases＆Shanghai Key Laboratory of Stomatology
2. Shanghai Stomatological Hospital, Fudan University; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University
- Publication Type:Journal Article
- Keywords:
Onlay graft;
bredigite;
β-tricalcium phosphate;
autogenous bone;
bone insufficiency;
implant;
3D printing;
bone scaffolds;
bone tissue engineering;
animal model;
bone regeneration
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2023;31(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To evaluate the osteogenic effects of using a 3D-printed bredigite(BRT) bone scaffold on the Onlay graft in rats.
Methods:BRT scaffold material was fabricated by 3D printing technology as the experimental group, β-tricalcium phosphate(β-TCP) bone scaffolds were used as the control group. The scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical test and in vitro degradation test. An animal model of Onlay graft in SD rats was established. According to the different grafts, the animal model was divided into autogenous bone transplantation group (Auto Group), β- TCP group and BRT group. At 12 weeks after the operation, the target line was obtained for gross observation, micro CT scanning and analysis, and HE staining histological analysis.
Results:BRT scaffolds have distinctive surface topographywith relatively uniformly sized pores in SEM imaging. A higher mechanical strength and degradation rate was obtained with BRT scaffolds（46.80 ± 3.44) MPa, compared to β-TCP scaffolds（11.29 ± 1.30) MPa (P<0.001）. At day 35 in vitro, the mass of BRT was degraded 27.18% ± 1.41%, and the Ca, Mg and Si ion concentrations were maintained at a high level in BRT group. Micro CT and histological quantitative results showed that the proportion of bone regeneration in BRT group was 16.83% ± 2.11% and 19.08% ± 2.17%, respectively, which was higher than that in the β- TCP group (8.48% ± 1.85%, 10.81% ± 1.33%) (P < 0.05).
Conclusion:3D-printed BRT scaffolds promote bone regeneration in the Onlay graft.
