Diagnostic Efficiency of Noninvasive Preimplantation Genetic Testing for Aneuploidy
- VernacularTitle:无创胚胎植入前非整倍体筛查的诊断有效性分析
- Author:
Lei JIA
1
;
Jing-bo CHEN
1
;
Ying-chun GUO
1
;
Shu-jing HE
1
;
Zhi-qiang ZHANG
1
;
Wen-long SU
1
;
Xiao GONG
2
;
Cong FANG
1
Author Information
1. Reproductive Medicine Research Center， The Sixth Affiliated Hospital of Sun Yat-sen University， Guangzhou 510655， China
2. Department of Epidemiology and Biostatistics， School of Public Health， Guangdong Pharmaceutical University， Guangzhou 510006， China
- Publication Type:Journal Article
- Keywords:
noninvasive preimplantation genetic testing for aneuploidy （niPGT-A）;
blastocyst culture medium/blastocoel fluid （BCM/BF）;
trophectoderm biopsy
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(1):73-80
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the diagnostic efficiency of noninvasive preimplantation genetic testing for aneuploidy （niPGT-A）. MethodsA total of 24 blastocysts diagnosed as aneuploid or mosaic by initial trophectoderm biopsy （TE1） preimplantation genetic testing for aneuploidy （PGT-A） were donated by 11 couples with reciprocal translocation or Robertsonian translocation. All the PGT cycles were performed at Center for Reproductive Medicine in the Sixth Affiliated Hospital of Sun Yat-sen University from July 2018 to July 2019. The 24 frozen-thawed blastocysts were reanalyzed by trophectoderm re-biopsy （TE2）， inner cell mass （ICM） biopsy and collection of spent blastocyst culture medium/blastocoel fluid （BCM/BF） using Next Generation Sequencing （NGS） platform. With the corresponding ICM result as the actual chromosomal status， the karyotype concordances with corresponding ICM were compared among TE1， TE2 and BCM/BF sampling. ResultsThe karyotype concordance rates of TE1， TE2 and BCM/BF with their corresponding ICM were 66.7%， 87.5% and 79.2%， respectively （P>0.05）. No statistically significant difference was found between any two groups （P>0.05）. ConclusionsniPGT-A by using BCM/BF could achieve similar diagnostic efficiency as TE2-biopsy PGT-A and ICM-biopsy PGT-A.
