Screening of Key Genes and Prediction of Drugs for Ischemic Stroke Based on Bioinformatics Approach
- VernacularTitle:基于生物信息学途径筛选缺血性脑卒中关键基因及药物预测
- Author:
Yu YU
1
;
Zhong-xing WANG
1
Author Information
1. Department of Anesthesiology，The First Affiliated Hospital of Sun Yat-sen University， Guangzhou 510080 ，China
- Publication Type:Journal Article
- Keywords:
ischemic stroke;
GSEA;
bioinformatics;
drugs prediction
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(1):42-50
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo screen out key genes and important signal pathways of ischemic stroke to predict potential drugs that might be useful for stroke. MethodsWe obtained GSE98319 Microarray data from GEO database which contained sequencing results from control （Sham group） and experimental （middle cerebral artery occlusion， MCAO） mice. Limma package of R language was used to analyze Microarray data. Differentially expressed genes were selected with P <0.05 and |log2FC|>0.6 as the criteria. Protein-Protein Interaction Network （PPIN） of differentially expressed genes was constructed by STRING online website and visualized by Cytoscape software. Quantitative real-time polymerase chain reaction（qRT-PCR）was performed to testify hub genes expression level. Gene Set enrichment analysis （GSEA） algorithm was used in functional enrichment analysis of Kyoto Encyclopedia of Genes and Genomics （KEGG） and Gene Ontology （GO） with FDR <0.05 as the enrichment standard. Finally， potential agents for stroke were predicted by Connectivity Map. ResultsA total of 521 differentially expressed genes were identified， 421 up-regulated and 100 down-regulated. Two hub genes： Drd4（P=0.000 019）and Hcar2（P=0.000 094）expression level upregulated in stroke mice cortex validated by qRT- PCR. Finally， four small molecule compounds：Esmolol， Methimazole， Cephaeline and Narciclasine were predicted to have potential therapeutic effects on ischemic stroke. ConclusionsDrd4 and Hcar2 may be closely related to the occurrence and development of ischemic stroke. Four potential therapeutic drugs are predicted， providing reference for the subsequent research on drug therapy.
