Establishment of a Method for Determination of Amygdalin in Quality Control System of Qingfei Paidu Granules
10.13422/j.cnki.syfjx.20220818
- VernacularTitle:清肺排毒颗粒质量控制体系中苦杏仁苷含量测定方法的建立
- Author:
Yan ZHANG
1
;
Yanyan ZHOU
1
;
Hongjie WANG
1
;
Lixin YANG
1
;
Haiyu ZHAO
1
;
Baolin BIAN
1
;
Huakai WU
2
;
Ling HAN
3
;
Nan SI
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
2. Zhangzhou PienTze Huang Pharmaceutical Co. Ltd.，Zhangzhou 363000，China
3. China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.，Shenzhen 518110，China
- Publication Type:Journal Article
- Keywords:
Qingfei Paidu granules;
almond;
content determination
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(14):127-133
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveAlmond， which is bitter in taste， contains traces of toxic substances. For the sake of the safety of prescriptions containing this medicinal material， the processing method of "soaking in boiling water" was selected. Moreover， through literature research and network pharmacology， the characteristic index of this medicinal material was determined. On this basis， a method was established for the determination of amygdalin in Qingfei Paidu Granules （QFPD） and the transfer rate of it in the processing of this prescription was monitored， aiming at improving the quality control system of QFPD. MethodThe high performance liquid chromatography conditions are as follows： YMC Triart C18 column （4.6 mm × 150 mm， 5 µm）， mobile phase of methanol-water with flow of 1.0 mL·min-1， column temperature of 35 ℃， and detection wavelength of 210 nm. ResultThe linear curve fitted well and the average recovery of amygdalin was 97.74% with RSD of 4.3%. The transfer rates of amygdalin from the medicinal material to the extract， from extract to concentrate， and from concentrate to granules were investigated with this method. The result showed that the average transfer rate from the medicinal material to the granules was （60±3.91）%. The comparison of transfer rate between the processes suggesting that the extraction of the medicinal material might be the key part influencing the prescription preparation. ConclusionThe method is simple， sensitive， reproducible， stable， and accurate， and the index is reasonable. Thus， the method can be used for the quality control of QFPD and determination of transfer rate of components in the preparation of QFPD. This study further improves the quality control standard of almond in QFPD， which can serve as a reference for the clinical application of QFPD.
