Xuebijing Injection Regulates Mitochondrial N-formyl Peptides/NLRP3 Inflammatory Pathway to Treat Severe Acute Pancreatitis in Rats
10.13422/j.cnki.syfjx.20220738
- VernacularTitle:血必净注射液调节线粒体N-甲酰肽/NLRP3炎症通路对重症急性胰腺炎大鼠模型的治疗机制
- Author:
Yi XIAO
1
;
Zhi-qiao FENG
2
;
Gui-xian ZHANG
3
;
Hong-sheng SHEN
3
;
Wen-chang LI
3
;
Xia LI
3
;
Rui-fang GAO
3
;
Hong-bin LIU
3
Author Information
1. Graduate School，Tianjin Medical University，Tianjin 300070，China
2. Tianjin Chase Sun Pharmaceutical Co. Ltd.，Tianjin 301700，China
3. Tianjin Institute of Medical and Pharmaceutical Science，Tianjin 300020，China
- Publication Type:Journal Article
- Keywords:
severe acute pancreatitis;
Xuebijing injection;
mitochondrial N-formyl peptides;
formyl peptide receptor 1;
nucleotide-binding oligomerization domain-like receptor 3;
Xuefu Zhuyutang
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(7):88-94
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the therapeutic effect of Xuebijing injection （XBJ） on sodium taurocholate （Na-Tc）-induced severe acute pancreatitis （SAP） in rats. MethodForty rats were randomly assigned into 5 groups： sham operation group， SAP model group， and low-， medium-， and high-dose （4， 8， 12 mL·kg·d-1， respectively） XBJ groups. SAP model was established by retrograde injection of Na-Tc （1 mL·kg-1） into the biliary and pancreatic ducts. XBJ was injected intraperitoneally 3 days before and 0.5 h after modeling. The ascitic fluid volume and the pancreas weight-to-body weight ratio were measured. The pathological changes of pancreatic tissue were observed via hematoxylin-eosin （HE） staining. The protein levels of formyl peptide receptor 1 （FPR1） and nucleotide-binding oligomerization domain-like receptor 3 （NLRP3） in pancreatic tissue were detected by immunohistochemistry. Western blot was employed to determine the expression levels of NADH-ubiquinone oxidoreductase chains 1-6 （MT-ND1， MT-ND2， MT-ND3， MT-ND4， MT-ND5， and MT-ND6） in rat plasma. ResultCompared with sham operation group， the SAP model group showcased increased ascitic fluid volume and pancreas weight-to-body weight ratio （P<0.05）， serious lesions in pancreatic tissue， increased total pathological score （P<0.05）， and up-regulated protein levels of FPR1 and NLRP3 in pancreatic tissue （P<0.05）. The model group had lower MT-ND2 level （P<0.05） and higher MT-ND1， MT-ND3， and MT-ND6 levels in plasma （P<0.05） than the sham operation group， while MT-ND4 and MT-ND5 had no significant differences between the two groups. Compared with SAP model group， the XBJ treatment decreased ascitic fluid volume and pancreas weight-to-body weight ratio （P<0.01）， ameliorated pancreatic lesions， and down-regulated the protein levels of FPR1 and NLRP3 in pancreatic tissue （P<0.01）. The treatments， especially high-dose XBJ （P<0.01）， down-regulated the expression of MT-ND1 （P<0.01）， MT-ND3 （P<0.01）， MT-ND6 （P<0.01）， and MT-ND4 and did not change that of MT-ND5. ConclusionXBJ may antagonize partial mitochondrial N-formyl peptides and excessive inflammatory response mediated by FPR1/NLRP3 to treat SAP in rats.
