Effects of NLRP3-mediated pyroptosis on olfaction dysfunction in allergic rhinitis.
10.3760/cma.j.cn115330-20210629-00383
- VernacularTitle:NLRP3介导的焦亡在变应性鼻炎嗅觉障碍中的作用研究
- Author:
Fang Wei ZHOU
1
;
Tian ZHANG
2
;
Ying JIN
2
;
Yi Fei MA
2
;
Zhi Peng XIAN
1
;
Zhi Min WU
1
;
Guo Dong YU
3
Author Information
1. Department of Clinical Medicine, Guizhou Medical University, Guiyang 550004, China.
2. Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China.
3. Department of Clinical Medicine, Guizhou Medical University, Guiyang 550004, China Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Caspases/therapeutic use*;
Disease Models, Animal;
Humans;
Inflammasomes/therapeutic use*;
Mice;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Ovalbumin;
Pyroptosis;
Rhinitis, Allergic/drug therapy*;
Smell
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2022;57(4):433-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the relationship between NLRP3-mediated pyroptosis and olfactory dysfunction (OD) in allergic rhinitis (AR), and to evaluate the therapeutic potential of CY-09, a selective NLRP3 inhibitor for OD. Methods: An AR mouse model was established with ovalbumin, and the olfactory function of AR mice was detected by the buried food pellet test. Mice with OD were intraperitoneally injected with CY-09 or saline. The activation of microglia and astrocytes in olfactory bulb was detected by immunohistochemistry. The expression level of pyroptosis associated protein was detected by Western blot. The level of pyroptosis associated proinflammatory factor mRNA was determined by real-time PCR. SPSS 24.0 software was used for statistical analysis. Results: After the test, ovalbumin successfully established AR mice model, in which 52.5% (21/40) of them showed OD. The number of activated microglia and astroglia in olfactory bulb tissue in OD group were more than those in non-OD group (all P<0.05). Compared with the control group, the expression of NLRP3, caspase-1 and gasdermin D (GSDMD) was significantly increased in the olfactory bulb of the OD group (all P<0.05). CY-09 could significantly reduce the level of NLRP3, caspase-1, GSDMD, IL-1β and IL-18 expression, and inhibite the activation of microglia and astrocytes in the olfactory bulb tissues (all P<0.05). Conclusion: NLRP3-mediated pyroptosis is closely related to the OD associated with AR. CY-09 could improve the olfactory function in AR mice, which may be related to blocking the NLRP3-mediated pyroptosis.
