Distributive characteristics of HBV DNA CpG islands in HBsAg positive mothers and its relationship with intrauterine transmission.
10.3760/cma.j.cn112338-20211010-00779
- VernacularTitle:HBsAg阳性母亲C基因型HBV DNA CpG岛分布特点和宫内传播的关系
- Author:
Ting WANG
1
;
Dan Dan WANG
1
;
Wen Xin CHEN
1
;
Cong JIN
1
;
Yan Di LI
1
;
Lin Zhu YI
1
;
Shu Ying FENG
2
;
Bo WANG
2
;
Yong Liang FENG
1
;
Su Ping WANG
1
Author Information
1. Department of Epidemiology/Center of Clinical Epidemiology and Evidence-Based Medicine, Shanxi Medical University, Taiyuan 030001, China.
2. Department of Obstetrics and Gynaecology, the Third People Hospital of Taiyuan City, Taiyuan 030001, China.
- Publication Type:Journal Article
- MeSH:
Biomarkers;
CpG Islands;
DNA, Viral/genetics*;
Female;
Hepatitis B;
Hepatitis B Surface Antigens;
Hepatitis B virus/genetics*;
Humans;
Infant, Newborn;
Infectious Disease Transmission, Vertical;
Mothers;
Pregnancy;
Pregnancy Complications, Infectious
- From:
Chinese Journal of Epidemiology
2022;43(5):728-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the type, length, and CG loci of HBV DNA CpG islands in HBsAg positive maternal C genotype and its relationship with intrauterine HBV transmission, so as to provide a new perspective for the study of intrauterine transmission of HBV. Methods: From June 2011 to July 2013, HBsAg-positive mothers and their newborns who delivered in the obstetrics and gynecology department of the Third People's Hospital of Taiyuan were collected. Epidemiological data were collected through face-to-face questionnaires and electronic medical records. Serum HBV markers and serum HBV DNA were detected by electrochemiluminescence and quantitative fluorescence PCR, respectively. Intrauterine transmission of HBV was determined by positive HBsAg and/or HBV DNA in femoral venous blood before injection of HBV vaccine/Hepatitis B immunoglobulin within 24 h of birth. A total of 22 mothers and their newborns with HBV DNA load ≥106 IU/ml in intrauterine transmission were selected as the intrauterine transmission group, and 22 mothers with HBV DNA load ≥106 IU/ml without intrauterine transmission were chosen as the control group by random seed method. The distribution prediction of CpG islands of HBV DNA in 39 mothers with genotype C by HBV DNA sequencing was analyzed. Results: Among 39 mothers with HBV C genotype, 19 were in the intrauterine transmission group, and 20 were in the control group. The HBV DNA of 39 patients with genotype C traditional CpG island Ⅱ and Ⅲ, while the control group had traditional CpG island Ⅰ and novel CpG island Ⅳ and Ⅴ. The length of CpG island Ⅱ and Ⅲ and the number of CG loci of CpG island Ⅱ in the intrauterine transmission group differed from those in the control group (P<0.05). The CpG island Ⅱ length ≥518 bp and the number of CG loci ≥40 in the intrauterine transmission group (11/19) were significantly higher than those in the control group (2/20) (P<0.05). The length of CpG island Ⅱ and the number of CG loci in the X gene promoter region (Xp region) were higher than those in the control group (P<0.05). In the HBV intrauterine transmission group, most of maternal (12/19) HBV DNA CpG island Ⅱ completely covered the Xp region, which was significantly higher than that in the control group (5/20), and the number of HBV DNA Xp region CG loci was higher than that in the control group (P<0.05). Conclusions: The distribution of maternal C genotype HBV DNA CpG islands is related to intrauterine transmission. The length of CpG island Ⅱ and the number of CG sites may increase the risk of intrauterine transmission of HBV.
