Urine metabolomics study of hepatocellular carcinoma.
10.3760/cma.j.cn112152-20200825-00765
- VernacularTitle:肝细胞癌尿代谢组学初步研究
- Author:
Xiu Feng XIE
1
;
Yan SUN
1
;
Xiao Hang ZHAO
1
Author Information
1. State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
- Publication Type:Journal Article
- Keywords:
Biomarker;
Hepatocellular neoplasms;
Non-invasive diagnosis;
Urinary metabolism
- MeSH:
Carcinoma, Hepatocellular/metabolism*;
Chromatography, High Pressure Liquid/methods*;
Humans;
Liver Neoplasms/metabolism*;
Mass Spectrometry/methods*;
Metabolomics/methods*
- From:
Chinese Journal of Oncology
2022;44(3):252-259
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the urinary small molecular metabolites and their metabolic characteristics of patients with hepatocellular carcinoma (HCC). Methods: High throughput ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to detect the small molecular metabolites in urine of healthy control (n=10), patients with hepatic hemangioma (n=10) and patients with HCC (n=10). The orthogonal projections to latent structures-discriminant analysis (OPLS-DA), hierarchical cluster analysis of multivariate analysis and univariate analysis were used to analyze the differential metabolites of the three groups. Results: The metabolic profiles of the three groups showed that the total of 381 differential metabolites were identified and divided into 96 up-regulated metabolites and 285 down-regulated metabolites. There were 55 urinary metabolites specifically related to HCC. Twenty-one of them were significantly up-regulated, including Acetyl-DL-Leucine, Ala Asp, HoPhe-Gly-OH, while 34 were significantly down-regulated, including Selenocystathionine, Met Trp Met Cys, Valsartan acid and so on. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the differential metabolites were mainly enriched in glutamine/glutamate metabolism, lysine biosynthesis, tricarboxylic acid cycle and purine metabolism. Conclusions: The occurrence of HCC is accompanied by the abnormalities of multiple metabolites and metabolic pathways. The analysis of the characteristic metabolic profile of urine in patients with HCC is helpful to find metabolic markers and potential therapeutic targets for liver cancer.
