Activation-induced markers for evaluation of specific CD4 + T cell immune response in HIV-1-infected patients
10.3760/cma.j.cn112309-20210409-00114
- VernacularTitle:使用活化诱导标记法评价HIV-1感染者特异性CD4 +T细胞免疫应答的研究
- Author:
Qianying WANG
1
;
Tiantian WANG
;
Shuo WANG
;
Hong PENG
;
Kunxue HONG
;
Yiming SHAO
;
Dan LI
Author Information
1. 中国疾病预防控制中心性病艾滋病预防控制中心 传染病预防控制国家重点实验室，北京 102206
- Keywords:
Flow cytometry;
Activation-induced markers;
Intracellular cytokine staining;
CD4 + T lymphocytes
- From:
Chinese Journal of Microbiology and Immunology
2022;42(1):23-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method using activation-induced markers (AIM) to detect the function of HIV-1-specific CD4 + T cell subsets for evaluating the immune response of HIV-1-specific CD4 + T cells more effectively. Methods:Twelve chronically HIV-1-infected patients without antiviral therapy and six healthy people without HIV-1 infection were enrolled in this study. The function of HIV-1-specific T lymphocytes was detected by AIM and ICS based on polychromatic flow cytometry. The performance of the two methods in assessing HIV-1-specific CD4 + T cell immune response in HIV-1-infected patients was evaluated. Results:The positive rates of HIV-1-specific PD-1 + CD25 + CD4 + T, CD69 + CD200 + CD4 + T, CD69 + ICOS + CD4 + T, CD69 + ICOS + CD8 + T、CD137 + CD69 + CD8 + T、PD-1 + CD25 + CD8 + T and OX40 + PD-1 + CD8 + T cells in all of the HIV-1 patients were 11/12, 8/12, 7/12, 8/12, 8/12, 7/12 and 7/12 using AIM method. ICS results showed that the positive rates of HIV-1-specific IL-2 + CD4 + T, IFN-γ + CD4 + T, TNF-α + CD4 + T, IFN-γ + CD8 + T, TNF-α + CD8 + T and IL-2 + CD8 + T cells were 2/12, 2/12, 0, 12/12, 10/12 and 5/12, respectively. Conclusions:AIM method was more sensitive in antigen-specific CD4 + T cell detection, and could be used as a complementary method to ICS in assessing antigen-specific T cell response.
