Development of a novel multi-functional integrated bioconjugate effectively targeting K-Ras mutant pancreatic cancer
- Author:
Yang-Yang WANG
1
;
Liang LI
;
Xiu-Jun LIU
;
Qing-Fang MIAO
;
Yi LI
;
Meng-Ran ZHANG
;
Yong-Su ZHEN
Author Information
1. Department of Pediatric Oncology,Tianjin Medical University Cancer Institute and Hospital,Tianjin,300060,China;NHC Key Laboratory of Biotechnology of Antibiotics,Department of Oncology,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,100050,China
- Keywords:
Pancreatic cancer;
Folate receptor;
Multi-functional;
Macropinocytosis-enhanced;
Bioconjugate;
K-Ras;
PEGylation
- From:
Journal of Pharmaceutical Analysis
2022;12(2):232-242
- CountryChina
- Language:Chinese
-
Abstract:
Folate receptor(FR)overexpression occurs in a variety of cancers,including pancreatic cancer.In addi-tion,enhanced macropinocytosis exists in K-Ras mutant pancreatic cancer.Furthermore,the occurrence of intensive desmoplasia causes a hypoxic microenvironment in pancreatic cancer.In this study,a novel FR-directed,macropinocytosis-enhanced,and highly cytotoxic bioconjugate folate(F)-human serum albumin(HSA)-apoprotein of lidamycin(LDP)-active enediyne(AE)derived from lidamycin was designed and prepared.F-HSA-LDP-AE consisted of four moieties:F,HSA,LDP,and AE.F-HSA-LDP presented high binding efficiency with the FR and pancreatic cancer cells.Its uptake in wild-type cells was more extensive than in K-Ras mutant-type cells.By in vivo optical imaging,F-HSA-LDP displayed prominent tumor-specific biodistribution in pancreatic cancer xenograft-bearing mice,showing clear and lasting tumor localization for 360 h.In the MTT assay,F-HSA-LDP-AE demonstrated potent cytotoxicity in three types of pancreatic cancer cell lines.It also induced apoptosis and caused G2/M cell cycle arrest.F-HSA-LDP-AE markedly suppressed the tumor growth of AsPc-1 pancreatic cancer xenografts in athymic mice.At well-tolerated doses of 0.5 and 1 mg/kg,(i.v.,twice),the inhibition rates were 91.2％and 94.8％,respectively(P＜0.01).The results of this study indicate that the F-HSA-LDP multi-functional bioconjugate might be effective for treating K-Ras mutant pancreatic cancer.
