Simultaneous determination of ten phenols in Polygoni Cuspidati Rhizoma by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry
10.3760/cma.j.cn115398-20201123-00188
- VernacularTitle:超高效液相色谱-四极杆-飞行时间质谱法同时测定虎杖中10种酚类物质含量
- Author:
Min WANG
1
;
Jie XU
;
Jing WANG
;
Na YANG
;
Hang LIU
;
Weihong GE
Author Information
1. 南京大学医学院附属鼓楼医院药学部，南京 210008
- Keywords:
Polygoni Cuspidati Rhizoma (TCD);
Ultra-performance liquid chromatography- quadrupole/time- of-flight mass spectrometry;
Active constituent;
Phenols
- From:
International Journal of Traditional Chinese Medicine
2022;44(1):61-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to determinate ten phenols (polydatin, resveratrol, rhein, emodin, chrysophanol, physcion, oxyresveratrol, 2,3,5,4'-tetera-hydroxystilbene-2-O-β-D-glucoside, (＋)-catechin and (-)-epicatechin) in Polygoni Cuspidati Rhizoma simultaneously based on the high-resolution multiple reaction monitoring (MRMHR) mode of ultra- performance liquid chromatography- quadrupole/time-of-flight mass spectrometry. Methods:The assay was performed on Waters ACQUITY UPLC BEH C18 (2.1 mm× 100 mm, 1.7 μm) column using acetonitrile-0.1% formic acid as the mobile phase. The flow rate was 0.2 ml/min. The MRMHR mode was adopted for quantification.Results:The analyzed compounds showed good linearity relationships ( r2>0.999). The intra- and inter-batch relative standard deviations were all <5% and the recovery rate was between 96.28%-103.23%. The content of polydatin was the highest, followed by resveratrol and emodin. However, the contents of chrysophanol and oxyresveratrol were relatively low and some batches were unqualified. The contents of analyzed compounds varied significantly among the ten batches. Conclusion:The proposed UPLC-Q/TOF-MS method was successfully established to determinate ten phenols in Polygoni Cuspidati Rhizoma simultaneously, which could provide technical support for the quality evaluation of Polygonum cuspidatum.
