Research progress on the detection methods and their application in ferroptosis
10.16438/j.0513-4870.2022-0276
- VernacularTitle:铁死亡主要检测方法及其应用的研究进展
- Author:
Shu-hua OUYANG
1
;
Yan-ping WU
1
;
Wan-yang SUN
1
;
Chang-yu YAN
1
;
Hiroshi KURIHARA
1
;
Yi-fang LI
1
;
Rong-rong HE
1
Author Information
1. Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
- Publication Type:Research Article
- Keywords:
ferroptosis;
etection method;
fluorescence probe;
liquid chromatography-mass spectrometry;
lipid peroxidation
- From:
Acta Pharmaceutica Sinica
2022;57(6):1544-1556
- CountryChina
- Language:Chinese
-
Abstract:
Ferroptosis is a novel type of cell death, which is distinguished from the traditional cell death pathways such as apoptosis, proptosis, necrosis and autophagy in terms of morphology, biochemistry and genetics. The main features of ferroptosis are the iron accumulation and lipid peroxidation. The regulation mechanism of ferroptosis involves glutathione metabolism, lipid peroxidation reactions and iron metabolism, which are closely related to the pathological process of tumor, aging, neurodegenerative diseases, ischemia reperfusion injury, cardiovascular and cerebrovascular diseases, kidney injury, hepatic fibrosis and so on. How to effectively study the role of ferroptosis regulation mechanism in the treatment of diseases becomes the hot spot and focus of the ferroptosis research. In recent years, with the in-depth study of ferroptosis, the identification, confirmation and the mechanism of ferroptosis have been developed significantly and have come forth continuously, in the meantime, techniques based on the morphology, biochemistry, molecular biology and genetics have been widely applied in the detection of ferroptosis. In order to deepen readers' understanding of ferroptosis and its detection methods, this paper will mainly review the current research progress on the detection methods and their application in ferroptosis, summarize and discuss their advantages and disadvantages in the detection of ferroptosis, this knowledge are crucial for better understanding and studying the biological function of ferroptosis.
