The Correlation Analysis between the Titer of IgG Anti-A/B Erythrocyte Antibody In Vivo of the Neonate and the Severity of Hemolytic Disease of Newborn.
10.19746/j.cnki.issn.1009-2137.2022.02.038
- Author:
Jian SONG
1
;
Ting YANG
1
;
Xiao-Fang ZHOU
1
;
Yin-Zhong YANG
2
;
Duan-Liang PENG
1
;
Jian-Bo ZHANG
1
Author Information
1. Department of Clinical Laboratory, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610071, Sichuan Province, China.
2. Department of Clinical Laboratory, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610071, Sichuan Province, China,E-mail:121892126@qq.com.
- Publication Type:Journal Article
- Keywords:
ABO blood group incompatibility;
antibody titer;
clinical prognostic factor;
haemolytic disease of newborn
- MeSH:
ABO Blood-Group System;
Bilirubin;
Blood Group Incompatibility;
Erythroblastosis, Fetal;
Erythrocytes;
Female;
Hematologic Diseases;
Hemolysis;
Humans;
Immunoglobulin G;
Infant, Newborn;
Pregnancy;
Retrospective Studies
- From:
Journal of Experimental Hematology
2022;30(2):547-551
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the titer of IgG anti-A/B erythrocyte antibody in vivo of the neonate with hemolytic disease of newborn(HDN), and explore its clinical valua in evaluating the severity of HDN.
METHODS:300 neonates with HDN, 50 neonates with neonatal hyperbilirubinemiain and 50 healthy neonates were selected as research object and Microtubes Gel Test was used to detect the titer of IgG anti-A/B erythrocyte antibody in vivo. Their clinical data and their mothers' prenatal examination data were retrospectively analyzed. Three hemolysis tests (direct antiglobulin test, free antibody test and release test), irregular antibody screening, and the titer of IgG anti-A/B blood group antibody was determined by serological method. Red blood cells(RBC), hemoglobin(Hb), reticulocytes(Ret) and nucleated red cells were detected by hematology analyzer. Indirect bilirubin and albumin(Alb) were detected by biochemical analyzer. The relationship between the titer of IgG anti-A/B erythrocyte antibody in vivo and the severity of HDN was analyzed.
RESULTS:There were six serological diagnosis modes in the HDN group,the difference between modes was statistically significant (P<0.05). The antibody titer relationship between HDN neonates and pregnant women was positive correlation(r=0.8302). The highest antibody titer of release test and free antibody test were 1∶32 and 1∶2, and the difference was statistically significant（P<0.05）. RBC, Hb and Alb in HDN patients were lower than those in neonatal hyperbilirubinemia patients and healthy neonates (P<0.05), and were negatively relevant with antibody titer in vivo (r=-0.8016). Bilirubin content in HDN patients were higher than those in neonatal hyperbiliru binemia patients and healthy neonates group(P<0.05), and was positively relevant with antibody titer in vivo (r=0.8731). The hospital day in HDN patients was significantly relevant with the antibody titer in vivo (r=0.8547), but not with the age, sex, weight and ABO blood types (P>0.05).
CONCLUSION:The detection of antibody titer in HDN patients can be used to evaluate the antibody concentration in vivo, predict the ability of antibody to induce erythrocyte hemolysis, and help to judge the serenrity and prognosis of HDN.
