Analysis of chemical constituents in classical prescription Danggui Buxue Decoction based on RP-Q-TOF-MS and HILIC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20210330.301
- Author:
Dong-Mei LEI
1
;
Chang-Liang YAO
2
;
Xue-Bing CHEN
2
;
Jian-Qing ZHANG
2
;
Jia-Yuan LI
2
;
De-An GUO
1
Author Information
1. School of Chinese Materia Medica, Nanjing University of Chinese Medicine Nanjing 210023, China Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica,Chinese Academy of Sciences Shanghai 201200, China.
2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica,Chinese Academy of Sciences Shanghai 201200, China.
- Publication Type:Journal Article
- Keywords:
Danggui Buxue Decoction;
UPLC-Q-TOF-MS;
chemical constituents;
classical prescription;
hydrophilic interaction chromatography
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/chemistry*;
Prescriptions;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2022;47(8):2109-2120
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of classical prescription Danggui Buxue Decoction were analyzed by reversed-phase(RP) chromatography and hydrophilic interaction chromatography(HILIC) coupled with quadrupole time-of-flight mass spectrometry. RP separation of Danggui Buxue Decoction was performed on ACQUITY UPLC HSS T3(2.1 mm×100 mm, 1.8 μm), while HILIC separation was on Waters BEH Amide(2.1 mm×100 mm, 1.7 μm). Mass spectrometry(MS) data were acquired in both negative and positive ion modes. Chemical constituents of Astragali Radix and Angelicae Sinensis Radix were searched from Reaxys and thus the in-house library was established. MS data were further analyzed by MassLynx 4.1 combined with in-house library, HMDB, Reaxys, and comparison with reference substances. In conclusion, a total of 154 compounds were identified and characterized: 16 saponins, 44 flavonoids, 10 phthalides, 7 phenylpropanoids, 15 bases and the corresponding nucleosides, 30 oligosaccharides, and 32 other compounds. Among them, 65 compounds were detected by HILIC-MS/MS. This study provides experimental evidences for the material basis research, quality control, and preparation development of Danggui Buxue Decoction and a reference method for comprehensive characterization of Chinese medicine decoctions typified by classical prescriptions.
