6-Gingerol ameliorates adipose tissue insulin resistance in aging rats.
10.19540/j.cnki.cjcmm.20211008.702
- Author:
Quan-Cao ZHAN
1
;
Yu-Zhe LIU
2
;
Yu-Meng XI
1
;
Yan ZENG
2
;
Jia-Yu TIAN
1
;
Li LIU
2
;
Dong LI
1
;
Yamahara JOHJI
3
;
Jian-Wei WANG
1
Author Information
1. Chongqing Key Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical University Chongqing 400016, China.
2. Faculty of Basic Medical Sciences,Chongqing Medical University Chongqing 400016, China.
3. Kyoto Institute of Food and Drug Research Kyoto 602-8136, Japan.
- Publication Type:Journal Article
- Keywords:
6-gingerol;
adiponectin;
adiponectin receptor 1;
inflammation;
insulin resistance
- MeSH:
Adipose Tissue;
Aging;
Animals;
Catechols;
Fatty Alcohols;
Insulin Resistance;
Male;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2022;47(6):1642-1649
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effects and mechanisms of 6-gingerol on adipose tissue insulin resistance in naturally aging rats with glycolipid metabolism disorders. Twenty-seven aging male SD rats were randomly divided into a model group(aged, n=9) and two groups treated with 6-gingerol at 0.05 mg·kg~(-1)(G-L, n=9) and 0.2 mg·kg~(-1)(G-H, n=9). Six young rats were randomly assigned to a normal control group(NC). Rats were treated for seven weeks by gavage. Non-esterified fatty acid(NEFA) and insulin content was determined by enzyme-linked immunosorbent assay(ELISA), and adipose tissue insulin resistance index(Adipo-IR) was calculated. HE staining was used to observe the size of adipocytes in epididymal white adipose tissue(eWAT). The gene and protein expression levels of adiponectin receptor 1(AdipoR1), AMP-activated protein kinase α(AMPKα), phosphorylated AMPK(p-AMPKα~(Thr172)), peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α), phosphatidylinositol 3-kinase(PI3 K), protein kinase B(Akt), phosphorylated Akt(p-Akt~(Ser473)), tumor necrosis factor-α(TNF-α), c-Jun N-terminal kinase 1/2(JNK1/2), phosphorylated JNK1/2(p-JNK~(Thr183/Tyr185)), interleukin-1β(IL-1β), and interleukin-6(IL-6) in adiponectin(APN), insulin, and inflammatory factor signaling pathways were detected by Western blot and real-time RCR, respectively. The results showed that 6-gingerol at a high dose could significantly decrease the fasting plasma content of NEFA and insulin and reduce Adipo-IR. Additionally, 6-gingerol at a high dose significantly increased the protein and mRNA expression of APN, AdipoR1, PGC-1α, and PI3 K in eWAT, elevated the relative expression of p-AMPK~(Thr172) and p-Akt~(Ser 473), reduced the protein and mRNA expression of TNF-α, IL-1, and IL-6 in eWAT, and decreased the relative expression of p-JNK1 and p-JNK2. This study reveals that 6-gingerol can improve insulin sensitivity of adipose tissues in aging rats with glycolipid metabolism disorders, and this effect is presumedly achieved by enhancing the PI3 K/Akt signaling pathway, inhibiting adipose tissue inflammation, increasing APN synthesis, enhancing AdipoR1 expression, and activating its downstream AMPK/PGC-1α signaling pathway.
