Effect of wheat-grain moxibustion on the expression of Beclin-1/GRP78 in spinal dorsal horn in rats with cervical spondylotic radiculopathy.
10.13703/j.0255-2930.20210404-k0006
- Author:
Yuan-Yuan LIN
1
;
Sheng-Yong SU
2
;
Yi-Yang XU
1
;
Hui-Qian CAI
1
;
Xi ZHANG
1
;
Mei-Xiang QIN
1
;
Fang-Xing JIANG
1
;
Xin-Ying LIN
1
;
Shan-Na PAN
1
Author Information
1. First Clinical Medical College of Guangxi University of CM, Nanning 530001, China.
2. Department of Acupuncture and Moxibustion, First Affiliated Hospital of Guangxi University of CM, Nanning
- Publication Type:Journal Article
- Keywords:
Beclin-1;
GRP78;
autophagy;
cervical spondylotic radiculopathy (CSR);
endoplasmic reticulum stress;
wheat-grain moxibustion
- MeSH:
Animals;
Beclin-1/genetics*;
Endoplasmic Reticulum Chaperone BiP;
Moxibustion;
RNA, Messenger;
Radiculopathy/therapy*;
Rats;
Rats, Sprague-Dawley;
Spinal Cord;
Spinal Cord Dorsal Horn;
Spondylosis;
Triticum/genetics*
- From:
Chinese Acupuncture & Moxibustion
2022;42(5):533-539
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of wheat-grain moxibustion at "Dazhui" (GV 14) on the expressions of Beclin-1 and GRP78 in spinal dorsal horn in rats with cervical spondylotic radiculopathy (CSR), and to explore the possible analgesic mechanism of wheat-grain moxibustion for CSR.
METHODS:A total of 48 SD rats were randomly divided into a sham operation group, a model group, a wheat-grain moxibustion group and a wheat-grain moxibustion+3-MA group, 12 rats in each group. The CSR model was prepared by spinal cord insertion method. Three days after modeling, the rats in the model group were intraperitoneally injected with 1 mL of 0.9% sodium chloride solution; the rats in the wheat-grain moxibustion group were treated with wheat-grain moxibustion at "Dazhui" (GV 14, 6 cones per time) on the basis of the model group; the rats in the wheat-grain moxibustion+3-MA group were intraperitoneally injected with 3-MA solution and wheat-grain moxibustion at "Dazhui" (GV 14, 6 cones per time). The three groups were intervened for 7 days, once a day. The gait score and mechanical pain threshold were observed before treatment and 7 days into treatment; after the treatment, the expressions of mRNA and protein of Beclin-1 in spinal dorsal horn were detected by real-time fluorescence quantitative PCR and immunohistochemistry; the expression of GRP78 protein in spinal dorsal horn was detected by Western blot method; the autophagosomes and ultrastructure in spinal dorsal horn neurons were observed by electron microscope.
RESULTS:After the treatment, compared with the sham operation group, in the model group, the gait score was increased and the mechanical pain threshold was decreased (P<0.01), and the expression of GRP78 protein in spinal dorsal horn was increased (P<0.01). Compared with the model group and the wheat-grain moxibustion+3-MA group, in the wheat-grain moxibustion group, the gait score was decreased and mechanical pain threshold was increased (P<0.01), and the expression of GRP78 protein in spinal dorsal horn was decreased, and the expressions of mRNA and protein of Beclin-1 were increased (P<0.01). Under electron microscope, the ultrastructure of spinal dorsal horn neurons in the wheat-grain moxibustion group was not significantly damaged, and its structure was basically close to normal, and the number of autophagosomes was more than the other three groups.
CONCLUSION:Wheat-grain moxibustion at "Dazhui" (GV 14) has analgesic effect on CSR rats. The mechanism may be related to moderately up-regulate the expression of Beclin-1, enhance autophagy and reduce endoplasmic reticulum stress.
