Study on the fecal metabolomics of CUMS-susceptible and CUMS-resilient rats
10.16438/j.0513-4870.2021-1707
- VernacularTitle:应激敏感与应激抵抗大鼠粪便代谢组学研究
- Author:
Sen-yan WANG
1
;
Fu-xiao WEI
1
;
Huan-le LIU
1
;
Si-jun ZHAO
2
;
Xue-mei QIN
1
;
Xiao-jie LIU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China; Key Laboratory of Effective Substances Research and Utilization in Traditional Chinese Medicine of Shanxi Province, Shanxi University, Taiyuan 030006, China
2. Shanxi Institute for Food and Drug Control, Taiyuan 030001, China
- Publication Type:Research Article
- Keywords:
epression;
chronic unpredictable mild stress;
stress susceptibility;
stress resilience;
sucrose preference rate;
fecal metabolomics
- From:
Acta Pharmaceutica Sinica
2022;57(5):1411-1419
- CountryChina
- Language:Chinese
-
Abstract:
Proton nuclear magnetic resonance (1H NMR) based metabolomics was applied to characterize the fecal metabolic profiles of chronic unpredictable mild stress (CUMS)-depression (CUMS-D) and CUMS-resilience (CUMS-R) rats. The fecal biomarkers and metabolic pathways involved in CUMS-D and CUMS-R were screened and identified, revealing the underlying mechanisms of two different responses of the body to the same stresses. Firstly, the classic depression model, i.e. CUMS, was constructed. According to the fecal metabolomics profiles, the model rats were divided into two groups, i.e. the CUMS-D group and the CUMS-R group. And then, the depression statuses of CUMS-D rats and CUMS-R rats were verified by their sucrose preference rates. Lastly, multivariate data analysis was applied to clarify the fecal biomarkers and corresponding metabolic pathways involving in CUMS-D and CUMS-R. The results show that compared with the control rats, the sucrose preference rates of CUMS-D rats were significantly reduced. By contrast, the sucrose preference rates of CUMS-R rats had no significant difference. At the same time, CUMS-D and CUMS-R showed both unique and shared biomarkers and pathways. Three pathways are significantly related to CUMS-D, including taurine and hypotaurine metabolism, alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. Glycerolipid metabolism and tryptophan metabolism are specific pathways related to CUMS-R. This study explores the mechanisms of the emergence of susceptible and resilience of rats under the same stimulus from a metabolomics perspective. The current findings provide not only a new perspective for studying depression, and personalized and precision treatments in clinic, but also the research and development of antidepressants.
