Brain Cell Injuries and Behavioral Changes in Rats with Kernicterus
10.3969/j.issn.1006-9771.2016.06.004.
- VernacularTitle:核黄疸模型大鼠脑细胞损伤及行为学后遗症的研究①
- Author:
Yi LIU
1
,
2
;
Nong XIAO
1
,
2
;
Zhensha MAO
1
,
2
Author Information
1. Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development with Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China;
2. Department of Rehabilitation in Children's Hospital of Chongqing Medical University, Chongqing 400014, China
- Publication Type:Journal Article
- Keywords:
kernicterus, gait analysis, beam-walking test, Morris water maze test, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(6):640-644
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the brain cell injuries and behavioral changes of newborn rats with kernicterus. Methods Twenty-five 5-day-old Sprague-Dawley rats were divided into control group (n=11) and model group (n=14) radomly. The model group was injected with bilirubin solution 10 μg/g in the cisterna magna, while the control group was injected with equal volume of normal saline. The neurobehavioral changes were observed and the body mass were recorded. TUNEL staining was used to check the apoptosis of striatal nerve cells of basal ganglia in the model group (n=3) on the first day after modeling. The remaining rats were assessed by gait analysis and beam-walking test 19 days after birth, and Morris water maze test was performed 30 days after birth. Results The model group showed apparently abnormal neurobehavioral changes, such as clenched fists, opisthotonos and the body mass were significantly lower in the model group than in the control group (F>27.707, P<0.001). TUNEL staining showed striatal nerve cells apoptosis in the model group. For the gait analysis, the step lengths of both hind legs were shorter (t>4.129, P<0.01), and the difference of step length was longer (t=-4.415, P<0.001) in the model group than in the control group, however, there was no significantly difference in the step width between two groups (t=0.462, P=0.649). For the beam-walking test, the score was lower in the model group than in the control group (t=-3.644, P=0.004). For the Morris water maze test, the escape latency was longer (F>6.206, P<0.05), and the number of crossing platform was less (t=3.297, P=0.004) in the model group than in the control group. Conclusion The newborn rats' model of kernicterus showed deficits in multiple motor functions and learning and memory ability, which could be assessed by gait analysis, beam-walk test and Morris water maze test, respectively.
