Study on the laboratory historical control values of biological indicators in SD rats with 28-day repeated dose oral toxicity tests
10.11763/j.issn.2095-2619.2021.03.002
- Author:
Li-hai ZENG
1
;
Jie-wei ZHENG
1
;
Zhi-wei XIE
1
;
Feng-rong LU
1
;
Guo-liang LI
1
;
Hai-lan WANG
1
Author Information
1. Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment Guangzhou,Guangdong 510300,China
- Publication Type:Journal Article
- Keywords:
SD rats;
Body mass;
Blood routine;
Blood biochemical index;
Organ mass;
Organ coefficient;
Reference value
- From:
China Occupational Medicine
2021;48(03):247-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the laboratory historical control values for biological indicators in SD rats with 28-day repeated dose oral toxicity tests. METHODS: The body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of 10 batches of specific pathogen free SD rats in the control group and the control additional group were collected for 28-day repeated dose oral toxicity tests, and the historical control values was established. RESULTS: The body mass of both male and female SD rats increased with the increasing age(all P<0.01). The body mass of male rats was higher than that of female rats each week(all P<0.01). The body mass, blood routine and serum biochemical indexes, organ mass and organ coefficient of SD rats were affected by the age and gender of rats to varying degrees. The effects of age and gender on organ mass and organ coefficient were not consistent. The laboratory historical control values of body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of SD rats were established according to the age measured in weeks and the gender of rats. CONCLUSION: The laboratory control values of biological indicators of SD rats should be established according to different weekly age and the gender of rats. Organ coefficient is more suitable as an observation index for toxicological safety evaluation compared with organ mass.
