Quality evaluation of Aster souliei based on quantitative analysis of multi-components by single marker
10.16438/j.0513-4870.2021-1173
- VernacularTitle:基于一测多评法的缘毛紫菀多成分含量测定及质量评价
- Author:
Xue-yan SU
1
;
Ying-xiu ZHANG
1
;
Ri-za ZHAO
1
;
Wen-pu CAI
1
;
Zhi-feng ZHANG
2
Author Information
1. College of Pharmacy and Institute of Tibetan Plateau, Southwest Minzu University, Chengdu 610041, China
2. College of Pharmacy and Institute of Tibetan Plateau, Southwest Minzu University, Chengdu 610041, China; Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory, Chengdu 610041, China
- Publication Type:Research Article
- Keywords:
italic>Aster souliei;
quantitative analysis of multi-components by single marker;
relative correction factor;
chlorogenic acid;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2022;57(3):775-782
- CountryChina
- Language:Chinese
-
Abstract:
To establish a quantitative analysis of multi-components by single marker (QAMS) for the determination of Aster souliei Franch., the relative correction factors (fx) of neochlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin, apigenin and kaempferol were established by ultra-high performance liquid chromatography with chlorogenic acid as internal reference. Meanwhile, the content of each component was determined by the external standard method (ESM) and QAMS, and a linear regression model was established to verify the feasibility and accuracy of the QAMS. Hierarchical clustering analysis (HCA) and orthogonal partial least square discriminate analysis (OPLS-DA) were used to evaluate the quality of 23 batches of A. souliei. The results showed that the repeatability of each fx was good. The average content of neochlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin, apigenin and kaempferol in 23 batches of A. souliei by QAMS was 0.165, 0.234, 6.115, 0.478, 0.484, 3.359, 1.382, 0.210, 0.172, and 0.057 mg·g-1, respectively. The mean content determined by the ESM method was 0.163, 0.235, 6.172, 0.479, 0.483, 3.343, 1.413, 0.207, 0.171, and 0.056 mg·g-1. The results of HCA and OPLS-DA analysis show that 23 batches of A. souliei can be divided into two groups based on caffeic acid content. The content of the first group was between 0.873 to 5.647 mg·g-1, while the second was between 8.524 to 16.705 mg·g-1. This QAMS method can be used to simply and quickly evaluate the quality A. souliei.
