Anti-tumor activity of novel targeting HER2 antibody-drug conjugates
10.16438/j.0513-4870.2021-0789
- VernacularTitle:新型靶向HER2抗体MIL40偶联药物的抗肿瘤活性研究
- Author:
Jing GENG
1
;
Yue-mei YANG
2
;
Xin-ying LI
3
Author Information
1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2. Beijing Aegle Stem Technology Co., Ltd., Beijing 102604, China
3. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing 100850, China
- Publication Type:Research Article
- Keywords:
human epidermal growth factor receptor 2;
tumor targeting therapy;
antibody-drug conjugate;
DM1;
MMAE
- From:
Acta Pharmaceutica Sinica
2022;57(3):695-699
- CountryChina
- Language:Chinese
-
Abstract:
Antibody-drug conjugates (ADCs) are widely used in cancer treatment. Human epidermal growth factor receptor-2 (HER2) is overexpressed in various types of solid tumors and is a validated therapeutic target for cancers. To develop a more effective therapy, we generated a novel anti-HER2 humanized monoclonal antibody MIL40 and MIL40 drug conjugates as novel cancer therapies. The MIL40 was conjugated with small molecule cytotoxic agents DM1 [emtansine, N2'-deacetyl-N2'-(3-mercapto-1-oxopropyl)-maytansine] or monomethylauristatin E (MMAE) to generate ADCs, which were evaluated for their in vitro and in vivo anti-cancer activities. Experimental results show that MIL40-DM1 and MIL40-MMAE can effectively identify and bind to HER2-positive tumor cells. The binding capabilities of MIL40-DM1 and MIL40-MMAE with HER2 extracellular domain (ECD) antigens were not different after conjugation with DM1 or MMAE. The ADCs showed potent cytotoxicity in HER2-positive ovarian cancer cells SKOV3, breast cancer cells SKBR3 and stomach cancer cells N87 in vitro. MIL40-DM1 can effectively inhibit the volume and weight growth of SKOV3 transplant tumors in mice. The mice in this study were used and treated by following the international guidelines for the care and use of laboratory animals, and approved by Animal Ethics Committee of Institute of Military Cognitive and Brain Sciences.
