Mechanism study of Curcumin on inhibiting experimental choroidal neovascularization in vitro by AKT/HIF-1α/VEGF pathway
10.3980/j.issn.1672-5123.2022.4.03
- VernacularTitle:姜黄素通过AKT/HIF-1α/VEGF信号通路在体外抑制脉络膜新生血管的机制
- Author:
Shui-Ling Chen
1
Author Information
1. 1Department of Ophthalmology, Xiyuan Hospital of China Academy of Traditional Chinese Medicine, Beijing 100091, China|2Eye Hospital of China Academy of Traditional Chinese Medicine, Beijing 100040, China
- Publication Type:Journal Article
- Keywords:
choroidal neovascularization;
Curcumin;
vascular endothelial growth factor(VEGF);
hypoxia-inducible factor-1α(HIF-1α);
protein kinase B(AKT);
human retinal pigment epithelial cells;
human umbilical vein endothelial cells
- From:
International Eye Science
2022;22(4):541-548
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect and mechanism of curcumin on inhibiting choroidal neovascularization(CNV)in vitro. METHODS: Human retinal pigment epithelial(ARPE-19)cells chemical hypoxia model was established by cobalt chloride(CoCl2). CCK-8 method was used to detect the effect of curcumin on the activity of ARPE-19 cells induced by CoCl2. RT-qPCR and Western blot were used to detect the expression of AKT, HIF-1α, VEGF mRNA and protein in ARPE-19 cells hypoxia model induced by CoCl2. Cell scratch test, transwell chamber migration test, transwell chamber invasion test and matrigel matrix hose lumen formation test were used to observe the effects of conditioned medium of curcumin in ARPE-19 cells on the proliferation, migration, invasion and lumen formation of human umbilical vein endothelial cells(HUVEC)in non-contact condition. RESULTS:Chemical hypoxia model of ARPE-19 cells can successfully establish by CoCl2 at 100μmol/L. CoCl2 at the final concentration of 100μmol/L can promote the expression of AKT, HIF-1α and VEGF mRNA and p-AKT, HIF-1α and VEGF protein in ARPE-19 cells. Curcumin at the final concentration of 100μmol/L can reduce the expression of AKT, HIF-1α and VEGF mRNA in ARPE-19 hypoxia model. Curcumin at the final concentration of 100μmol/L can reduce the expression of AKT, HIF -1α and VEGF proteins in ARPE-19 hypoxia model. The conditioned medium of low(6.25μmol/L), medium(25μmol/L)and high dose(100μmol/L)curcumin in ARPE-19 cells can significantly inhibit the level migration of HUVEC. The conditioned medium in high dose group can significantly inhibit the vertical migration and cell invasion of HUVEC. The conditioned medium of middle and high dose curcumin in ARPE-19 cells can inhibit the lumen formation of HUVEC. CONCLUSION:Curcumin at 100μmol/L can protect ARPE-19 cells from hypoxia induced by CoCl2. Curcumin can inhibit the formation of blood vessels at the cellular level.
- Full text:202204003.pdf
