Analysis on ingredients and metabolites of Gegen Decoction absorbed into blood based on UHPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20210727.203
- Author:
Fang HONG
1
;
Yan YAN
2
;
Lei ZHAO
3
;
Bo-Yang YU
1
;
Cheng-Zhi CHAI
3
Author Information
1. Jiangsu Provincial Key Laboratory for Traditional Chinese Medicine Evaluation and Translational Research, China Pharmaceutical University Nanjing 211198, China Research Center for Traceability and Standardization of Traditional Chinese Medicines, China Pharmaceutical University Nanjing 211198, China.
2. Jiangsu Provincial Key Laboratory for Traditional Chinese Medicine Evaluation and Translational Research, China Pharmaceutical University Nanjing 211198, China.
3. Jiangsu Provincial Key Laboratory for Traditional Chinese Medicine Evaluation and Translational Research, China Pharmaceutical University Nanjing 211198, China Department of Pharmacology and Traditional Chinese Medicine, School of Chinese Materia Medica, China Pharmaceutical University Nanjing 211198, China.
- Publication Type:Journal Article
- Keywords:
Gegen Decoction;
UHPLC-Q-TOF-MS;
female Wistar rats;
plasma components
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Female;
Mass Spectrometry;
Paeonia;
Rats;
Rats, Wistar
- From:
China Journal of Chinese Materia Medica
2021;46(22):5944-5952
- CountryChina
- Language:Chinese
-
Abstract:
This study analyzed the plasma components of Gegen Decoction(GGD) by ultra-high-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS), which is expected to serve as a reference for exploring the pharmacodynamic substances of GGD. Female Wistar rats were given(ig) GGD and then plasma samples were collected and analyzed by UHPLC-Q-TOF-MS. The results showed that 42 chemical components were identified: 25 prototypes(14 from Puerariae Lobatae Radix, 6 from Glycyrrhizae Radix et Rhizoma, 3 from Paeoniae Radix Alba, and 2 from Ephedrae Herba) and 17 metabolites(from isoflavonoids in Puerariae Lobatae Radix and Glycyrrhizae Radix et Rhizoma). UHPLC-Q-TOF-MS was employed to achieve rapid analysis of plasma components of GGD, laying a basis for elucidating the therapeutic material basis and mechanism of GGD.
