Standardization and normalization of flow cytometry
10.3760/cma.j.cn114452-20210920-00586
- VernacularTitle:流式细胞分析的规范化和标准化
- Author:
Chenxue QU
1
;
Jianzhong WANG
Author Information
1. 北京大学第一医院检验科，北京 100034
- Keywords:
Flow cytometry;
Standardization;
Normalization
- From:
Chinese Journal of Laboratory Medicine
2021;44(12):1105-1108
- CountryChina
- Language:Chinese
-
Abstract:
Flow cytometry has been widely used in clinical practice. Due to the powerful user-defined function of flow cytometry, different instrument settings among manufacturers and lack of standard materials, the comparability of results needs to be improved and flow cytometry is facing the great challenge of normalization and standardization. In this paper, the recent progress of normalization and standardization about flow cytometry was discussed form the aspects of standardizing operation protocol, including specimen and centrifugation, standardizingantibody selection and panel combination and ensuring instrument and data analysis consistency.
