Comparative study of rapid paraffin section versus frozen section of histopathological evaluations of donor organ
10.3760/cma.j.cn421203-20201203-00407
- VernacularTitle:快速石蜡切片与冷冻切片在供器官组织病理学评估中的比较研究
- Author:
Jianlin CHEN
1
;
Xinqiang WANG
;
Jing XU
;
Jipin JIANG
;
Gang CHEN
;
Hui GUO
Author Information
1. 华中科技大学同济医学院附属同济医院器官移植研究所 器官移植教育部重点实验室 国家卫生健康委员会器官移植重点实验室 中国医学科学院器官移植重点实验室，武汉 430030
- Keywords:
Donated organ;
Histopathology;
Freezing microtomy;
Paraffin section
- From:
Chinese Journal of Organ Transplantation
2021;42(11):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the advantages and disadvantages of frozen section versus rapid paraffin section in the evaluations of donor organ.Methods:Five cases of donor liver and 8 cases of discarded donor kidney were collected from 2017 to 2021.Tissues were harvested and prepared by frozen section, rapid paraffin section and normal paraffin section.After hematoxylin-eosin (H&E) staining, the specimens of donor kidney/liver were evaluated by differential histopathological structures and donor quality scoring system.Results:Rapid paraffin section was similar to normal paraffin section in reflecting the proportion of glomerulosclerosis (18.6%±22.3%), arteriolar hyaline degeneration (43.7%±23.8%) and arteriolar stenosis (47.9%±29%). The proportion of glomerulosclerosis (0.8%±2.2%), arteriolar hyaline degeneration (4.9%±7.4%) and arteriolar stenosis (5.3%±7.5%) were lower in frozen sections than those in rapid paraffin sections.The diagnoses of hydropic degeneration and necrosis in donor liver were more accurate in rapid paraffin section.Conclusions:Rapid paraffin section is superior to frozen section in observing histopathological changes under microscope.Scoring of donor organ is more precise according to rapid paraffin section.
