LncRNA BBOX1-AS1 regulates radiosensitivity of ovarian cancer cells via miR-185-5p/RHOA
10.3760/cma.j.cn.115807-20210709-00214
- VernacularTitle:LncRNA BBOX1-AS1通过miR-185-5p/RHOA调节卵巢癌细胞放疗敏感性
- Author:
Hui LI
1
;
Yuansheng LEI
;
Shuangxue LI
;
Feng LI
;
Jieyun LEI
Author Information
1. 山西省人民医院妇科，太原 030012
- Keywords:
BBOX1-AS1;
miR-185-5p;
RHOA;
Ovarian cancer;
Radiosensitivity
- From:
Chinese Journal of Endocrine Surgery
2021;15(5):499-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential mechanism and effects of LncRNA BBOX1-AS1 in radiosensitivity of ovarian cancer.Methods:qRT-PCR was used to explore BBOX1-AS1, miR-185-5p expression in OC tissue and cells. Dual luciferase reporter assay was used to confirm the interaction of BBOX1-AS1, miR-185-5p and RHOA. MTT assay and flow cytometry were used to detect the proliferation and apoptosis of OC cells.Results:BBOX1-AS1 was up-regulated in OC tissue and cells, compared with the cell proliferative activity at 2, 3, 4 day (0.89±0.07) (1.48±0.13) (1.69±0.15) in si-NC group, proliferative activity in si-BBOX1-AS1 group (0.59±0.06) (0.97±0.09) (1.21±0.10) was obviously down-regulated (all P<0.05) . Compared with si-NC group (6.24±0.28) , silencing of BBOX1-AS1 induced apoptosis (12.07±1.33) (all P<0.05) . miR-185-5p was down-regulated in OC tissue and cells, the targeting relationship between BBOX1-AS1 and miR-185-5p, RHOA and miR-185-5p was confirmed. Inhibition of miR-185-5p reversed the effects of BBOX1-AS1 on OC cells (all P<0.05) . Conclusion:LncRNA BBOX1-AS1 inhibits radiotherapy sensitivity of OC cells via regulating miR-185-5p/RHOA axis.
