Determinating the content of iridoid glycosides and flavonoids constitutents simultaneously in Xiaoer-Ganyan granules by UHPLC-PDA
10.3760/cma.j.cn115398-20200708-00103
- VernacularTitle:超高效液相色谱法同时测定小儿肝炎颗粒中环烯醚萜苷和黄酮类成分含量
- Author:
Honggao CHU
1
;
Qian TANG
;
Juanjuan LI
Author Information
1. 四川省广元市中心医院药学部 628000
- Keywords:
Xiao Er Gan Yan Ke Li;
Ultra-performance liquid chromatography;
Iridoid glycosides;
Flavonoids;
Assay (TCD);
Chemical compositions (TCD);
Quality control
- From:
International Journal of Traditional Chinese Medicine
2021;43(7):668-673
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an UHPLC-PDA method for determinating the content of 7 iridoid glycosides and 4 flavonoids constitutents simultaneously in Xiaoer-Ganyan granules. Methods:To take Agilent Ecilipse C18 (2.1 mm × 100 mm, 1.6 μm), and the colunm temperature was at 30 ℃. The mobile phase consists of acetonitrile (A) and 0.05% phosphoric acid (B) in gradient elution at a flow rate of 0.3 ml/min. The detection wavelength was set at 240 and 278 nm.Results:The linear ranges of swertiamain, gentiopicrin, sweroside, shanzhiside methy ester, gardenoside, genipin-1-β-D-gentiobioside, geniposide, baicalin, wogonoside, baicalein and wogonin were 19.16-306.56 μg ( r=0.999 4), 3.34-53.28 μg ( r=0.999 1), 5.30-84.64 μg ( r=0.999 5), 0.80-12.80 μg ( r=0.999 4), 0.46-7.20 μg ( r=0.999 2), 2.78-44.48 μg ( r=0.999 6), 6.02-96.16 μg ( r=0.999 9), 33.22-531.36 μg ( r=0.999 9), 3.92-62.72 μg ( r=0.999 2), 2.38-37.92 μg ( r=0.999 7), 1.32-20.96 μg ( r=0.999 9), respectively. The average recoveries ( n=9) varied from 94.62%-107.53% with RSDs no more than 3.0%. Conclusion:Method validation suggested that the developed method was suitable for simultaneous determination of 11 major constitutents in Xiaoer-Ganyan granules, thus providing reference for the improvement of quality standard of the drug.
