In vitro and in vivo evaluation of folate-mediated triptolide exosome targeted delivery system against malignant melanoma
10.16438/j.0513-4870.2021-1478
- VernacularTitle:叶酸介导的雷公藤甲素外泌体靶向给药系统抗恶性黑色素瘤体内外评价
- Author:
Yong-wei GU
1
;
Liang-di JIANG
2
;
Yue DU
3
;
Ai-xue LI
2
;
Ji-yong LIU
1
Author Information
1. Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China
2. Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China; College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
3. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; Department of Pharmacy, Children's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200062, China
- Publication Type:Research Article
- Keywords:
triptolide;
exosome;
folic acid;
targeted drug delivery system;
malignant melanoma
- From:
Acta Pharmaceutica Sinica
2021;56(12):3224-3232
- CountryChina
- Language:Chinese
-
Abstract:
In this study, exosomes were extracted from human malignant melanoma cell A375. Folic acid (FA) receptor was used as target and triptolide (TPL) was used as model drug to prepare exosome targeted drug delivery system, FA-Exo/TPL. The physicochemical properties and antitumor effect were evaluated in vivo and in vitro. Gradient centrifugation method was applied to collect exosomes. Then, exosome was modified with FA for loading TPL. The particle sizes of the FA-Exo/TPL were about 100 nm with a double-layer membrane structure like a tray. It is characteristic of high encapsulation efficiency and drug loading. In vitro experiments showed that FA-Exo/TPL could be effectively uptaken by A375 cells, thus significantly inhibiting proliferation and promoting apoptosis the cells. In vivo experiment results showed that FA-Exo/TPL could effectively inhibit the growth of tumor tissue, prolong the model mice life cycle, and significantly reduce the systemic toxicity of the free drug, playing a synergistic and toxic role. Animal welfare and experimental procedures follow the regulations of the Animal Ethics Committee of Fudan University Shanghai Cancer Center. This study provides a new strategies and methods for the preparation of TPL against malignant melanoma.
