Identification and expression analysis of the DaNAC transcription factor family in Dipsacus asper
10.16438/j.0513-4870.2021-0899
- VernacularTitle:川续断DaNAC基因家族成员的鉴定及表达分析
- Author:
Jiao XU
1
;
Zheng-ping HU
1
;
Hua HE
1
;
Juan GUO
2
;
Wei-ke JIANG
1
;
Cheng-hong XIAO
1
;
Liang-yuan LI
1
;
Tao ZHOU
1
Author Information
1. Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
italic>Dipsacus asper;
italic>NAC transcription factor;
expression analysis;
asperosaponin Ⅵ
- From:
Acta Pharmaceutica Sinica
2021;56(12):3325-3330
- CountryChina
- Language:Chinese
-
Abstract:
italic>NAC transcription factor genes play an important role in regulating plant adversity stress tolerance and secondary metabolism. To explore DaNAC transcription factor participation in the synthesis of asperosaponin Ⅵ in Dipsacus asper, we analyzed the expression of DaNAC genes based on full-length transcriptome data from different tissues (root, stem, leaf, flower, seed) to provide a theoretical foundation for regulating the metabolism of D. asper. RNA-seq data was used to identify open reading frames. Bioinformatic methods were used to identify the conserved domain motifs and construct an evolutionary tree. qRT-PCR was carried out to analyze tissue-specific and adversity-stressed expression. Twenty-nine DaNAC sequences were identified, all of which contain the conserved NAM domain and conserved motif 1 and motif 2 at the N terminal. Five DaNAC genes are closely related to the NAC genes in Arabidopsis thaliana and rice that are involved in adversity stress and are clustered in the Group Ⅰ subfamily. qRT-PCR revealed that DaNAC genes are differentially expressed between tissues. The expression levels were highest in leaves, followed by roots, stems and petioles, and the lowest in flowers and seeds. Compared with normal growth conditions, the expression of four NAC genes was up-regulated by treatment with low temperature (15 ℃). The expression of three genes (34564NAC2, 33883NAC48, 6727NAC14) was up-regulated and one gene (34480NAC22) was down-regulated by 150 μmol·L-1 MeJA. The results illustrate that the expression of NAC genes is induced by adversity stress, which provides a foundation for further study on the role of NAC family members in adversity stress in D. asper.
