Comparative analysis of chemical constituents in hawthorn leaves from different sources
10.16438/j.0513-4870.2021-0884
- VernacularTitle:不同来源山楂叶化学成分比较分析
- Author:
Wei ZHENG
1
;
Ming ZHOU
2
;
Shuang-yan WANG
1
;
Qi LI
1
;
Jie ZHANG
1
;
Ye ZHAO
3
;
Xiao-hui ZHENG
3
;
Bao-lin GUO
4
;
Bai-ping MA
1
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
3. College of Life Sciences, Northwest University, Xi'an 710069, China
4. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
hawthorn leaf;
ifferent sources;
UHPLC-Q-TOF/MSE;
identification of chemical constituents;
ifferential analysis
- From:
Acta Pharmaceutica Sinica
2021;56(12):3526-3539
- CountryChina
- Language:Chinese
-
Abstract:
We identified and analyzed the components and chemical constituents of hawthorn leaves of Crataegus pinnatifida Bge. (wild) and C. pinnatifida Bge. var major N. E. Br (cultivated) by using ultra high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MSE) combined with UNIFI data analysis platform and multivariate statistics. Fifty-eight chemical compounds were identified, including organic acids, flavonoids, triterpenoic acids, monoterpenes and sesquiterpenoids; among them, terpenoid content was the most abundant. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to identify the differential components of hawthorn leaves from two sources. The results show that there are differences in the chemical compositions of the two sources, including 24 flavonoids and terpenoids (including monoterpenoids, sesquiterpenoids and triterpenoid acids). The types of flavonoids (such as rutin, vitexin-2''-O-rhamnoside, isovitexin-2''-O-rhamnoside, hyperoside, quercetin-3-O-β-D-glucoside) and terpenoids (crataegolic acid, corosolic acid and ursolic acid) in C. pinnatifida were more varied than those found in C. pinnatifida Bge. var major N. E. Br, and their contents were relatively higher. This study provides a comprehensively analysis of the different chemical components of hawthorn leaves from two sources listed in the Chinese Pharmacopoeia, and provides a basis for the selection of raw materials and the potential development and utilization of hawthorn leaves.
