Analysis and Identification of Chemical Constituents in Hypoglycemic Effective Fractions of Longan Folium Based on UPLC-Q-Orbitrap HRMS
10.13422/j.cnki.syfjx.20201752
- VernacularTitle:基于UPLC-Q-Orbitrap HRMS技术的龙眼叶降血糖有效部位化学成分分析
- Author:
Guang-qiang HUANG
1
;
Jie LIANG
1
;
Jin-yu WEI
1
;
Dong-fang HUANG
1
;
Jing LIN
1
;
Xiao-si CHEN
1
;
Xian-fu LIU
2
Author Information
1. Guangxi Zhuang Yao Medicine Engineering Technology Research Center， Guangxi University of Chinese Medicine，Nanning 530200，China
2. Faculty of Chinese Medicine Science，Guangxi University of Chinese Medicine，Nanning 530222，China
- Publication Type:Research Article
- Keywords:
Longan Folium;
hypoglycemic;
material basis;
ethyl acetate fraction;
n-butanol fraction;
ethanol fraction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(6):127-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Chemical constituents in hypoglycemic effective fractions of Longan Folium were isolated and identified by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap HRMS） to clarify the hypoglycemic substance basis of Longan Folium. Method:Chemical constituents in hypoglycemic effective fractions of Longan Folium were isolated on a Thermo Hypersil GOLD C18 column （2.1 mm×100 mm， 1.9 μm）， the mobile phase was 0.1% formic acid acetonitrile solution and 0.1% formic acid solution （containing and 10 mmol ammonium acetate） for gradient elution. HRMS was operated in the positive and negative ion modes with the scanning range of m/z 100-1 500. Result:The secondary fragment ion information of target compounds was selected and compared with the compounds reported in the databases and related literature to further confirm these compounds. Nine compounds were identified in the ethanol fraction of Longan Folium， including cynaroside， kaempferol， quercitrin， luteolin， shikimic acid， citric acid， L-tyrosine， adenosine and nicotinamide. A total of 11 compounds were determined in the ethyl acetate fraction （cynaroside， quercitrin， kaempferol， luteolin， shikimic acid， gallic acid， protocatechuic acid， adenosine， nicotinamide， L-phenylalanine and scopoletin）， and 10 compounds were identified in the n-butanol fraction （cynaroside， kaempferol-3-O-rutinoside， kaempferol， astragalin， luteolin， citric acid， gallic acid， adenosine， nicotinamide and 5-hydroxymethylfurfural）. And five common compounds were identified in these three hypoglycemic effective fractions. Conclusion:The established UPLC-Q-Orbitrap HRMS can quickly identify chemical constituents in three hypoglycemic effective fractions of Longan Folium， their main chemical constituents are flavonoids and their glycosides， organic acids and nitrogen-containing compounds， which provides technical support and scientific evidence for the study on pharmacodynamic material basis and quality control of Longan Folium.
