Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ1-42
10.13422/j.cnki.syfjx.20210802
- VernacularTitle:黄连解毒汤对Aβ1-42诱导AD大鼠学习记忆能力及胆碱能系统的影响
- Author:
Li-xin CAO
1
;
Cheng-jun DONG
1
;
Yan YANG
1
;
Yu-qing LIANG
1
;
Yu-xi LUO
1
;
Zhi-yan XIE
1
;
Jie-mei WANG
1
;
Bo XIA
1
;
Wen-bin WU
1
Author Information
1. Affiliated Hospital of Chengdu University of Traditional Chinese Medicine，Chengdu 610075，China
- Publication Type:Research Article
- Keywords:
Huanglian Jiedutang;
Alzheimer's disease;
cholinergic system;
α7 nicotinic acetylcholine receptor （α7nAChR）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(10):23-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Huanglian Jiedutang on learning and memory ability and the cholinergic system in Alzheimer's disease（AD） rats induced by amyloid β-protein（Aβ）1-42. Method:Sixty male SD rats were divided into normal group， model group， huperzine A group （2.1×10-5 g·kg-1）， high-， medium- and low dose of Huanglian Jiedutang groups （6，3，1.5 g·kg-1）. AD rat model was replicated by hippocampal injection of Aβ1-42. After 4 weeks of treatment， Morris water maze test was performed. Hematoxylineosin （HE） staining was used to observe the pathological changes of rat hippocampus. Sampling blood from abdominal aorta was taken. Acetylcholine （ACh）， acetylcholinesterase （AchE） and choline acetyltransferase （ChAT） in serum and hippocampus were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of hippocampal α7 nicotinic acetylcholine receptor （α7nAChR） protein was detected by Western blot. The expression of hippocampal α7nAChR mRNA was detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. Result:Compared with the normal group， there were obvious pathological changes in the model group，such as neuron necrosis in the cerebral cortex，pyramidal cell or granular cell necrosis in the hippocampus，disorder of arrangement and inflammatory cell infiltration，prolonged escape latency，decreased escape platform times，decreased residence time in the effective area and swimming path in the effective area （P<0.05，P<0.01）. The contents of α7nAChR mRNA，ACh，AchE，ChAT，α7nAChR in the hippocampus decreased （P<0.01）. Compared with the model group，the escape latency of the middle dose group was shorter （P<0.05）， the escape platform times，the swimming path in the effective area and the residence time in the effective area increased （P<0.05，P<0.01）， the contents of serum ACh，ChAT， hippocampal AchE，ChAT and α7nAChR increased （P<0.05，）. The expression of hippocampal α7nAChR protein significantly increased （P<0.01）， the residence time of effective area in high dose group was prolonged （P<0.01）， the times of escape platform increased，and the contents of serum ACh，ChAT and hippocampal ACh，AchE，α7nAChR protein and α7nAChR mRNA increased （P<0.05）. Conclusion:Huanglian Jiedutang can significantly improve the learning and memory ability of AD rats induced by Aβ1-42，and its mechanism may be related to the improvement of cholinergic system damage and enhancement of cholinergic system function induced by Aβ1-42.
