Anti-tumor Effect of Oxymatrine on Non-small Cell Lung Cancer Cell Lines and Its Mechanism
10.13422/j.cnki.syfjx.20211396
- VernacularTitle:氧化苦参碱对非小细胞肺癌细胞抗肿瘤作用及机制
- Author:
Jiao LUO
1
;
Wei XIE
2
;
Kun-chi ZHANG
2
;
Jin WANG
3
;
Li ZHOU
1
Author Information
1. School of Clinical Medicine，Guizhou Medical University，Guiyang 550025，China
2. Shanghai Key Laboratory for Molecular Imaging，Shanghai University of Medicine & Health Sciences，Shanghai 201318，China
3. Guizhou Engineering Vocational College，Tongren 554300，China
- Publication Type:Research Article
- Keywords:
oxymatrine;
non-small cell lung cancer（NSCLC）;
cell proliferation;
Notch signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(13):43-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of oxymatrine（OM） on proliferation，migration， and invasion of non-small cell lung cancer（NSCLC） A549 and H1299 cells and to explore the possible mechanism. Method:A549 and H1299 cells were treated by OM of different concentrations（0， 1.0，2.0，4.0，8.0，16.0， 32.0， and 64.0 mmol·L-1） and the cell viability was detected by cell counting kit-8 （CCK-8） assay. Transwell invasion and wound healing assays were applied to determine the effect of OM of different concentrations （8.0，16.0， and 32.0 mmol·L-1） on the invasion and migration of A549 and H1299 cells. Western blot was adopted to detect the changes in the expression of proteins related to the Notch signaling pathway after the treatment by OM of different concentrations （8.0，16.0， and 32.0 mmol·L-1）. Result:Compared with the control，OM could inhibit the proliferation （P<0.05，P<0.01） and hinder the cell invasion and migration of A549 and H1299 cells （P<0.01） in a dose-dependent manner. The results of Western blot showed that OM（32.0 mmol·L-1） could effectively counteract the expression levels of Notch1 intracellular domain（NICD），transcriptional complex proteins ［TNF-alpha converting enzyme（TACE） and recombining binding protein suppressor of hairless（RBPSUH）］， and Hes family hairy and enhancer of split 1（Hes1） in A549 and H1299 cells. Conclusion:OM was capable of inhibiting the proliferation，migration， and invasion of A549 and H1299 cells and also hindering the expression of proteins related to Notch signaling pathway.
