Data Mining of Caryophylli Fols-containing Formula and Analysis of Its Mechanism in Treatment of Diarrhea
10.13422/j.cnki.syfjx.20202054
- VernacularTitle:含丁香方剂的数据挖掘及其治疗腹泻的作用机制分析
- Author:
Qing-yao CHEN
1
;
Liang-feng WANG
1
;
Hui-ting LI
2
;
Yao WANG
3
;
Xiao-li LIU
2
;
Jie XU
1
;
Ming YANG
1
;
Fang WANG
1
;
Xiao-fei ZHANG
1
Author Information
1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of TCM，Nanchang 330004，China
2. College of Pharmacy，Chengdu University of TCM，Chengdu 610075，China
3. College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，China
- Publication Type:Research Article
- Keywords:
data mining;
network pharmacology;
diarrhea;
Caryophylli Fols;
inflammation;
brain-gut interaction;
formulas
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(5):158-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on database mining， the high-frequency compatibility of Caryophylli Fols as the core in formulas for treating diarrhea was analyzed， and the network pharmacology was used to elucidate the mechanism of the core drug group containing Caryophylli Fols in the treatment of diarrhea. Method:The online database of traditional Chinese medicine （TCM） was intelligently crawled by Python 3.8.1 programming， and the compatibility rules of Caryophylli Fols were analyzed， and the TCM with support≥0.30， confidence≥0.90 and lift≥1.00 was set as the core drug group of Caryophylli Fols. The components were searched and screened by TCM Systems Pharmacology Database and Analysis Platform （TCMSP）， and the disease targets were collected in Therapeutic Target Datebase （TTD）， GeneCards and DisGeNET database with "Diarrhea" as the key word. The network diagram of "TCM-ingredients-potential targets" was constructed by Cytoscape 3.7.1 software， and the network of protein-protein interaction （PPI） was constructed by STRING 11.0. Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis of potential targets was analyzed by R language， and the components of the core drug group were preliminarily verified and evaluated by Discovery Studio Client 2016 software. Result:A total of 155 formulas containing Caryophylli Fols for treating diarrhea were screened， involving 54 TCMs. The analysis of association rules showed that Caryophylli Fols was strongly associated with Myristicae Semen， Glycyrrhizae Radix et Rhizoma， Aucklandiae Radix and Atractylodis Macrocephalae Rhizoma in the treatment of diarrhea. The core drug group composed of these five TCMs involved 119 kinds of TCM ingredients and 114 potential targets， of which 104 potential targets were distributed in the nervous system， and the key targets were tumor protein p53 （TP53）， transcription factor activator protein-1 （JUN）， mitogen-activated protein kinase 8 （MAPK8）， interleukin-6 （IL-6）， 90 kDa heat shock protein αA1 （HSP90AA1） and so on. GO enrichment analysis mainly involved biological processes such as the regulation of neurotransmitter levels， blood circulation， hormone-mediated signal pathway and regulation of chemical synaptic transmission. IL-17 signal pathway， helper T cell 17 （Th17） cell differentiation， epidermal growth factor receptor and salmonella infection in KEGG pathways were closely related to the treatment of diarrhea. Molecular docking showed that the key target protein had high affinity with quercetin， kaempferol and β-sitosterol. Conclusion:The multi-components， multi-targets and multi-pathways involved in the core drug group of Caryophylli Fols are closely related to inflammation and nervous system， so it is speculated that it may treat diarrhea by repairing intestinal shielding integrity and regulating the levels of neurotransmitters.
