Effect of Triptonide on Proliferation and Apoptosis of SHI-1 Cells
10.13422/j.cnki.syfjx.20210323
- VernacularTitle:雷公藤内酯酮对SHI-1细胞增殖及凋亡的影响
- Author:
Hui-jie LI
1
;
Kun HONG
1
;
Xiao-qiang LI
1
;
Li LIU
1
;
Yu-qing TAN
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Research Article
- Keywords:
acute monocyte leukemia （AML）;
triptonide （TN）;
SHI-1 cell line;
proliferation;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(3):72-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to investigate the effect of triptonide （TN） on proliferation， cell cycle， apoptosis and expressions of apoptosis-related proteins of human acute monocytic leukemia（AML） cell line SHI-1， and to explore its possible mechanism of action. Method:The thiazolyl blue （MTT） colorimetric assay was applied to detect the inhibitory effect of 20，40，80，160，320 nmol·L-1 triptonide on the proliferation of SHI-1 cells for 48， 72 h. Changes in SHI-1 cell cycle before and after triptonide treatment were detected by flow cytometry propidium iodide （PI） simple staining， and changes in SHI-1 cell apoptosis before and after triptonide treatment were detected by flow cytometry with AnnexinV/PI double staining. Western blot was applied to detect the protein expression of cysteine protease （Caspase）-3， Caspase-8 and nuclear transcription factor kappaB（NF-κB） in SHI-1 cells before and after treatment with 80， 160 nmol·L-1 triptonide. Result:Compared with the blank group， 40，80，160，320 nmol·L-1 triptonide significantly inhibited the proliferation of SHI-1 cells（P<0.01） in a dose-dependent manner for 48， 72 h， while 160， 320 nmol·L-1 triptonide induced apoptosis of SHI-1 cells（P<0.01） for 48， 72 h， and 160 nmol·L-1 triptonide could decrease the S phase ratio of SHI-1 cells（P<0.01）. In addition， compared with the blank group， 80，160 nmol·L-1 triptonide induced the downregulation of NF-κB significantly（P<0.01）， 160 nmol·L-1 triptonide induced the downregulation of Caspase-3， Caspase-8 significantly（P<0.01）. Conclusion:Triptonide can inhibit the proliferation and induce apoptosis in vitro of SHI-1 cells， which may be related to the reduction of the cells in S phase proportion by triptonide， and the downregulation of the expression levels of Caspase-3， Caspase-8 and NF-κB proteins.
