Investigation of Pharmacodynamic Material Basis and Mechanism of Qianyang Yuyin Granules Against Hypertensive Renal Damage Based on LC/Q-TOF-MS and Network Pharmacology
10.13422/j.cnki.syfjx.20202047
- VernacularTitle:基于LC/Q-TOF-MS整合网络药理学探讨潜阳育阴颗粒防治高血压肾损害的药效物质基础与作用机制
- Author:
Xin-hua WU
1
;
Chang-yin LI
1
;
Yuan CAO
1
;
Hui QU
1
;
Yang HAN
1
;
Zhu-yuan FANG
1
Author Information
1. Affiliated Hospital of Nanjing University of Chinese Medicine，Nanjing 210029，China
- Publication Type:Research Article
- Keywords:
Qianyang Yuyin granules;
hypertensive renal damage;
network pharmacology;
liquid chromatography coupled with quadrupole time-of-flight mass spectrometry （LC/Q-TOF-MS）;
pharmacodynamic substances;
mechanism;
database
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(2):116-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To systematically study the chemical components of Qianyang Yuyin granules and explore its main pharmacodynamic substances and mechanism in the prevention and treatment of hypertensive renal damage. Method:Liquid chromatography coupled with quadrupole time-of-flight mass spectrometry （LC/Q-TOF-MS） was employed to comprehensively analyze the chemical components of Qianyang Yuyin granules. Agilent Poroshell 120 SB-C18 column （3.0 mm×100 mm， 2.7 μm） was used， flow rate was 0.4 mL·min-1， electrospray ionization （ESI） was applied and operated in positive and negative ion modes， the acquisition range was m/z 25-1 000. Mobile phase in positive ion mode consisted of water+10 mmol·L-1 ammonium formate+0.125% formic acid+0.1% methanol （A）-［acetonitrile-water （9∶1）+10 mmol·L-1 ammonium formate+0.125% formic acid］ （B）， and in negative ion mode consisted of water+10 mmol·L-1 ammonium formate+0.1% methanol （A）-［acetonitrile-water （9∶1）+10 mmol·L-1 ammonium formate］ （B） with the gradient elution （0-3.5 min， 5%B； 3.5-4 min， 5%-10%B； 4-9 min， 10%-25%B； 9-18 min， 25%-30%B； 18-25 min， 30%-50%B； 25-27 min， 50%-90%B； 27-32 min， 90%B； 32-33 min， 90%-5%B； 33-39 min， 5%B）. According to the information of the accurate mass， the multistage fragment ions， the mass spectrometric data of the standard substances and the relative reference literature， the structures of the chemical components in Qianyang Yuyin granules were identified. Based on the identified components， network pharmacology study， including target prediction and functional enrichment was applied to screen out the main active substances against hypertensive renal damage， and explore the potential mechanism. Result:A total of 99 chemical components were identified， from which 43 active substances and 48 key targets were screened out. The key components contained kaempferol， quercetin， ferulic acid， luteolin， caffeic acid methyl ester， cinnamic acid， aloe-emodin， emodin， gallic acid， N-trans-feruloyltyramine， isoorientin， 8-O-feruloylharpagide， ethyl caffeate， isookanin， cyasterone， 2，3，5，4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside， loganin， alisol B-23-acetate and harpagide. The key targets included vascular endothelial growth factor A （VEGFA）， serine/threonine protein kinase 1 （AKT1）， Jun proto-oncogene （JUN）， etc. Conclusion:Qianyang Yuyin granules mainly exert the effects of removing heat from the liver， tonifying the kidney and removing blood stasis via modulation of vascular endothelium， angiogenesis， inflammatory reaction， oxidative stress， immune response and so on.
