Effect of Suanzaoren Tang on Learning-memory Function and NLRP3 Pathway in Sleep-deprived Rats
10.13422/j.cnki.syfjx.20202107
- VernacularTitle:酸枣仁汤防治睡眠剥夺性大鼠学习记忆功能变化及其对NLRP3通路的影响
- Author:
Fan GUO
1
;
Dong-nan WU
2
;
Ling LIU
1
;
Xi-qian YANG
2
;
Jie FANG
1
;
Ping WANG
2
Author Information
1. Department of Encephalopathy， Hubei Hospital of Traditional Chinese Medicine（TCM）， Wuhan 430065， China
2. Clinical College， Hubei University of TCM， Wuhan 430065， China
- Publication Type:Research Article
- Keywords:
Suanzaoren Tang;
Nod-like receptor 3 （NLRP3） inflammasome;
neuroinflammation;
learning-memory function;
sleep deprivation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(2):22-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Suanzaoren Tang in improving learning-memory of sleep-deprived rats based on Nod-like receptor 3 （NLRP3） inflammatome pathway. Method:The rats were randomly divided into normal control group， model group， Eszolam group（5.4×10-4 g·kg-1·d-1）， low-dose Suanzaoren Tang group（4.59 g·kg-1·d-1）and high-dose Suanzaoren Tang group （18.36 g·kg-1·d-1）. In addition to normal control group， other groups were used to constructed sleep-deprived model， which was concurrent with 30-day continuous drug administration. Water maze was used to evaluate the learning-memory function of rats； The mRNA and protein expressions of NLRP3， apoptosis-related speckle proteins （ASC）， aspartic acid-specific cysteine protease-1 （Caspase-1）， interleukin-1（IL-1） and IL-18 in the hippocampus of rats were detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） and Western blot. Result:Compared with control group， the incubation period of the platform， the total distance of swimming and the duration of first reaching the platform in model group were significantly increased （P<0.01）， while the number of platform crossings and the target quadrant time were decreased （P<0.01）. Compared with the model group， the incubation period， total swimming distance and the duration of first reaching the platform in low-dose Suanzaoren Tang group and high-dose Suanzaoren Tang group were decreased to different degrees （P<0.05，P<0.01）， while the number of platform crossings and the target quadrant time were increased significantly （P<0.05，P<0.01），but with no significant change in estazolam group. Compared with normal control group， mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1β， IL-18 in the hippocampus of the model group were significantly increased （P<0.05，P<0.01）. Compared with model group， mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1β and IL-18 in the hippocampus of the rats in low-dose Suanzaoren Tang group and high-dose Suanzaoren Tang group were all decreased to different degrees （P<0.05）. The mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1β and IL-18 in the hippocampus of Suanzaoren group also decreased， but with no significant change. Conclusion:Suanzaoren Tang can improve the learning-memory function of sleep-deprived rats， and its mechanism is related to the inhibition of NLRP3 inflammatome pathway in hippocampus and the alleviation of neuroinflammation.
