Research Progress on Screening Active Components from Medicinal Plants Based on Affinity Ultrafiltration Coupled with LC-MS Technology
10.13422/j.cnki.syfjx.20202348
- VernacularTitle:亲和超滤-液质联用技术在药用植物活性成分筛选中的应用研究进展
- Author:
Li YANG
1
;
Bo-yuan ZHAO
1
;
Chun-long LI
1
;
Ke-xin WANG
1
;
Ting ZHANG
1
;
Shang WANG
1
;
Rong-hua LIU
1
;
Jun-wei HE
1
Author Information
1. Jiangxi Synergistic Innovation Center of Modern Technology and Industrial Development of Traditional Ethnic Medicines，School of Pharmacy，Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Jiangxi University of TCM，Nanchang 330004，China
- Publication Type:Research Article
- Keywords:
affinity ultrafiltration;
liquid chromatography-mass spectrometry;
medicinal plants;
active components;
targeted;
traditional Chinese medicine （TCM）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(8):196-208
- CountryChina
- Language:Chinese
-
Abstract:
Medicinal plants have a long history and play an important role in the prevention and treatment of human diseases. However， the interaction between bioactive components and specific biological targets in many medicinal plants is still unclear. Conventional separation and activity evaluation model of chemical constituents from natural products was time-consuming and laborious， and cannot truly reflect the interaction between the natural conformation of compounds and receptors， so these methods could not meet the urgent needs of modern drug development. Affinity ultrafiltration coupled with liquid chromatography-mass spectrometry （AUF-LC/MS） is a rapid， efficient and precise method focused on identification of bio-components from natural products， it can make up for many shortcomings found in conventional discovery of bio-constituents. However， AUF-LC/MS technology also has some drawbacks in experiments. In this paper， the principle， characteristics and application of AUF-LC/MS in the screening of active ingredients from medicinal plants are systematically reviewed， and its development prospect is prospected， so as to provide scientific basis for rapid and targeted screening of active components from medicinal plants.
