Molecular Identification of 22 Species of Lauraceae by DNA Barcoding
10.13422/j.cnki.syfjx.20211019
- VernacularTitle:22种樟科植物DNA条形码分子鉴定
- Author:
Dan-yun XU
1
;
Hui-ju ZHANG
1
;
Ke-xin LI
1
;
Quan YANG
1
;
Hong-yang GAO
1
Author Information
1. Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Research Article
- Keywords:
Lauraceae;
DNA barcoding;
identification;
matK;
rbcL;
rpoB
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(16):159-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective:DNA barcodes suitable for Lauraceae plants were screened，and 22 Lauraceae plants were identified and classified. Method:The DNA of 22 species of Lauraceae was extracted and amplified by polymerase chain reaction（PCR） with different DNA barcoding primers，followed by electrophoresis and sequencing. Codon Code Aligner was used to proofread，splice， and remove the forward and reverse primer sequences. The sequence was imported into DNAMAN for multiple sequence alignment，and the base mutation sites were analyzed. The Kimura 2-Parameter（K2P） distance of different plants was calculated by MEGA，and the variation degree was analyzed according to the genetic distance. The phylogenetic tree was constructed based on the adjacency method. Result:Three pairs of DNA barcoding primers were used to amplify and sequence the DNA of 22 species of Lauraceae，and 20 species were identified by comparing the specific base sites of gene sequences. Conclusion:Four Litsea plants could be identified by matK， three Phoebe plants except for Cinnamomum validinerve by rbcL， and 20 Lauraceae plants by the combination of matK， rbcL， and rpoB，which provided a theoretical basis for the identification and development of Lauraceae plants. Among them，matK was predominant in the identification of Lauraceae plants，and the results also showed that the combination of sequences for plant identification could achieve a better result in DNA barcoding. This study investigated the genetic relationship between Lauraceae plants at the molecular level，aiming at providing a basis for the investigation，cultivation，development，protection， and utilization of medicinal plant resources.
