Establishment of UPLC-PDA Fingerprint of Baoyuantang Substance Benchmark and Identification of Its Chemical Constituents
10.13422/j.cnki.syfjx.20202346
- VernacularTitle:保元汤物质基准UPLC-PDA指纹图谱的建立及化学成分指认
- Author:
Huan-bo CHENG
1
;
Hui HU
1
;
Qing-an LI
1
;
Peng LIU
1
;
Jiang XU
2
;
Guang-wei ZHU
2
;
A-qian CHANG
2
Author Information
1. Jing Brand Chizhengtang Pharmaceutical Co. Ltd.，Hubei Provincial Engineering Technology Research Center of Traditional Chinese Medicine （TCM） Formula Granule， Hubei Provincial Key Laboratory for Quality and Safety of TCM Health Food，Huangshi 435100，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Baoyuantang;
ultra performance liquid chromatography （UPLC）;
fingerprint;
mass spectrometry （MS）;
ginsenosides;
famous classical formulas;
substance benchmark
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(7):16-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the fingerprint of Baoyuantang substance benchmark， and to analyze and identify the common peaks. Method:A total of 15 batches of Baoyuantang substance benchmark were prepared， ultra performance liquid chromatography-diode array detector method （UPLC-PDA） was used to establish the fingerprint of the substance benchmark， and the methodology was developed. The chromatographic conditions were as follows：ACQUITY UPLC BEH Shield C18 column （2.1 mm×100 mm， 1.7 μm）， mobile phase of 0.05% formic acid solution （A） and 0.05% formic acid acetonitrile solution （ B） for gradient elution （0-0.5 min， 5%-19%B； 0.5-6 min， 19%B； 6-10 min， 19%-27%B； 10-20 min， 27%-45%B； 20-20.1 min， 45%-95%B； 20.1-23 min， 95%B）， the flow rate of 0.4 mL·min-1， the column temperature of 30 ℃， the detection wavelength at 203 nm and 260 nm， and the injection volume of 2 μL. Similarity evaluation system of traditional Chinese medicine fingerprint （2012 edition） was used to establish the fingerprint and generate the control fingerprint. The chemical constituents of Baoyuantang substance benchmark were identified by comparison of standard substances and UPLC-electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） with full information tandem mass spectrometry （MSE） and scanning range of m/z 50-1 200. Result:The similarities of 15 batches of Baoyuantang substance benchmark were above 0.90 by comparing with the control fingerprint. There were 37 common peaks， 22 of which were identified through UPLC-ESI-MS/MS， including liquiritin， violanthin， ginsenoside Rg1， ginsenoside Rb1， ginsenoside Re and so on. These components were all from Astragali Radix， Ginseng Radix et Rhizoma， Zingiberis Rhizoma Recens and Glycyrrhizae Radix et Rhizoma. Conclusion:This method is accurate， stable and reliable， which will basically reflect the overall chemical composition characteristics of Baoyuantang， and it provides experimental basis for development of the granules of this famous classical formulas.
