Analysis on Content Differences of Artemisinin and Arteannuin B in Different Provenances of Artemisia annua Under Indoor Hydroponic Conditions
10.13422/j.cnki.syfjx.20211547
- VernacularTitle:不同种源黄花蒿室内水培条件下青蒿素和青蒿乙素含量差异分析
- Author:
Rui-yang CHENG
1
;
Wen-rui HE
2
;
Xiao-feng SHEN
3
;
Li XIANG
1
;
Yu LIANG
4
;
Ying MENG
5
;
Qian-wen LIU
1
;
Zhen-jie FAN
6
;
Wei SUN
1
;
Jiang XU
1
;
Shi-lin CHEN
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
2. Chengdu University of Traditional Chinese Medicine（TCM），Chengdu 611137，China
3. Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100193，China
4. Dali University，Dali 671003，China
5. Shandong University of TCM，Jinan 250399，China
6. Henan University of Science and Technology，Luoyang 471023，China
- Publication Type:Research Article
- Keywords:
Artemisia annua;
artemisinin;
arteannuin B;
hydroponics;
germplasm;
ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）;
longitude and latitude
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(20):145-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of artemisinin and arteannuin B in different Artemisia annua germplasms， compare the differences of the two compounds among different A. annua germplasm under the condition of hydroponic homogenization and explore the key factors affecting contents of principal compounds in different A. annua germplasms. Method:Seedlings from different A. annua germplasms were arranged randomly and fed in a hydroponic cultivation system. Contents of artemisinin and arteannuin B were detected by ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） with multi-reaction monitoring mode and ACQUITY UPLC® BEH C18 column （2.1 mm×100 mm， 1.7 μm）， mobile phase was water-acetonitrile （95∶5， containing 0.1% formic acid， A） and acetonitrile-water （95∶5， containing 0.1% formic acid， B） for gradient elution （0-3.5 min， 25%-1%A； 3.5-3.6 min， 1%-25%A； 3.6-5.0 min， 25%A）， the flow rate was set at 0.4 mL·min-1. The content differences of artemisinin and arteannuin B in different provenances of A. annua were detected and analyzed statistically. Result:The established method had high sensitivity and good separation. A significant difference of artemisinin and arteannuin B contents was observed in different germplasms under the same culture conditions， that is， under the constant temperature of 25 ℃ in hydroponics. The provenance with higher artemisinin content was Yunnan， and the content was 3 810.597 μg·g-1. The highest strain of arteannuin B was Shanxi provenance germplasm with the content of 1 691.747 μg·g-1. According to the content of artemisinin， the provenances were arranged as follows：Yunnan， Hainan， Hubei， Guangxi， Zhejiang， Shanxi， Beijing， Shandong， Heilongjiang， and Gansu province germplasms. Correlation analysis showed that there was a significant negative correlation between artemisinin content and latitude of A. annua， but there was no significant correlation between contents of artemisinin and arteannuin B and longitude. Conclusion:The contents of artemisinin and arteannuin B among different A. annua germplasms were significantly different under the same culture environment， and the dominant factors affecting biosynthesis and accumulation of artemisinin and arteannuin B in A. annua may be the genetic background， suggesting that germplasm improvement is the key factor to improve the medicinal quality of A. annua in subsequent cultivation.
