Mechanism of Qingke Pingchuan Granule in Treatment of Acute and Chronic Bronchitis Complicated with Chronic Obstructive Pulmonary Disease： An Exploration Based on Network Pharmacology
10.13422/j.cnki.syfjx.20211112
- VernacularTitle:基于网络药理学探讨清咳平喘颗粒治疗急慢性支气管炎合并慢性阻塞性肺疾病的作用机制
- Author:
Tian-quan WANG
1
;
Jun-ling CAO
2
;
Jin-tao HU
3
;
Ling-yan DONG
3
;
Jing-feng OUYANG
4
;
Mei-jiao LIN
3
Author Information
1. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 102488，China
2. Dongfang Hospital，Beijing University of Chinese Medicine，Beijing 100078，China
3. Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100700，China
4. Beijing Key Laboratory of Traditional Chinese Medicine Prevention and Treatment of Major Diseases，Medical Laboratory Center，China Academy of Chinese Medicine Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Qingke Pingchuan granule;
network pharmacology;
acute and chronic bronchitis;
chronic obstructive pulmonary disease （COPD）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(18):160-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential mechanism of Qingke Pingchuan granule in treating acute and chronic bronchitis complicated with chronic obstructive pulmonary disease （COPD） by network pharmacology. Method:The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） was retrieved to collect the active components of Qingke Pingchuan granule and predict the action targets， followed by the construction of component-target network using Cytoscape 3.8. GeneCards， Online Mendelian Inheritance in Man（OMIM）， and DrugBank were used to harvest disease targets， whose names were put into UniProt for standardization. The treatment targets of Qingke Pingchuan Granule against the two diseases were obtained based on Venn diagram， which were then imported into the STRING platform for constructing the protein-protein interaction （PPI） network. Following the gene ontology（GO） and Kyoto encyclopedia of genes and genomes（KEGG） pathway enrichment analysis based on MetaScape， the active component-common target-signaling pathway network of Qingke Pingchuan granule against acute and chronic bronchitis complicated with COPD was finally constructed. The accuracy of the target was confirmed by literature. Result:A total of 165 active components， 374 related targets， 512 disease-related targets， and 130 common targets were obtained. Among them， the 14 core therapeutic targets were further subjected to GO enrichment analysis， which yielded 390 biological processes， nine cell components， and 23 molecular functions. The KEGG pathway analysis revealed 22 signaling pathways. Conclusion:Qingke Pingchuan granule alleviates the diseases possibly by regulating such targets as vascular endothelial growth factor receptor 2（KDR）， transforming growth factor beta-1 （TGF-β1）， caveolin 1（CAV1）， hypoxia-inducible factor-1alpha（HIF-1α）， and interleukin-2（IL-2）， affecting the synthesis and transport of regulatory factors in cytoplasm， and controlling the cell proliferation and apoptosis.
