Identification of Chemical Constituents in Chaishi Tuire Granules by UPLC-ESI-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20210846
- VernacularTitle:基于UPLC-ESI-Q-TOF-MS/MS技术快速鉴定柴石退热颗粒的化学成分
- Author:
Li YANG
1
;
Feng-rui YUAN
2
;
Lan CAO
1
;
Ye-lin DUAN
1
;
Meng ZOU
1
;
Yong ZHANG
1
;
Jun-wei HE
1
Author Information
1. Jiangxi University of Chinese Medicine，Nanchang 330004，China
2. Sinopharm Group Guangdong Medi-world Pharmaceutical Co. Ltd.，Foshan 528000，China
- Publication Type:Research Article
- Keywords:
Chaishi Tuire granules;
ultra-performance liquid chromatography-electrospray/quadrupole time-of-flight tandem mass spectrometry （UPLC-ESI-Q-TOF-MS/MS）;
flavonoids;
phenylpropanoids;
terpenoids;
alkaloids;
fragmentation pattern
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):152-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To rapidly identify the chemical constituents of Chaishi Tuire granules by ultra-performance liquid chromatography-electrospray/quadrupole time-of-flight tandem mass spectrometry （UPLC-ESI-Q-TOF-MS/MS）. Method:Chromatographic separation was conducted on a Phenomenex® Luna omega C18 column （2.1 mm×100 mm， 1.6 μm） with 0.1% formic acid aqueous solution （A）-acetonitrile （B） as the mobile phases for gradient elution （0-20 min， 5%-40%B； 20-40 min， 40%-95%B； 40-43 min， 95%B）， the flow rate was set at 0.3 mL·min-1. MS data were collected in positive and negative ion modes， the scanning range was m/z 150-1 500 and electrospray ionization （ESI） was employed. The chemical constituents of Chaishi Tuire granules were identified by comparing with the retention time and the mass data of the reference substances， as well as the accurate mass， MS/MS fragment ions， mass spectrometry databases （PubChem， MassBank， ChemicalBook and others） and related literature. Result:A total of 85 chemical constituents were identified， including 28 flavonoids， 24 phenylpropanoids， 11 terpenoids， 10 alkaloids， 4 quinones， and 8 others. Among them， 19 constituents derived from Lonicerae Japonicae Flos， 14 constituents derived from Scutellariae Radix， 10 constituents derived from Isatidis Radix， 9 constituents derived from Taraxaci Herba， 9 constituents derived from Forsythiae Fructus， 4 constituents derived from Bupleuri Radix， 4 constituents derived from Anemarrhenae Rhizoma， and 4 constituents derived from Rhei Radix et Rhizoma. Conclusion:Chaishi Tuire granules is rich in phytochemicals， which are derived from many of traditional Chinese medicines. This study can lay a foundation for the quality control， material basis and in vivo metabolic analysis of this preparation.
