Capsaicin Induces Apoptosis of Colon Cancer SW480 Cells Through TRPV1
10.13422/j.cnki.syfjx.20211796
- VernacularTitle:辣椒碱通过TRPV1诱导结肠癌SW480细胞凋亡
- Author:
Xian-wei HUANG
1
;
Ge LI
1
;
Meng-wei YAO
1
;
Hong WEI
1
Author Information
1. Guizhou Medical University， Guiyang 550025，China
- Publication Type:Research Article
- Keywords:
capsaicin;
transient receptor potentia vanilloid 1（TRPV1）;
p53;
SW480 cells;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):75-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of capsaicin on colon cancer SW480 cells and the underlying molecular mechanism through the transient receptor potential vanilloid 1（TRPV1）. Method:Capsaicin groups with different concentrations and a blank group were set up. The cell viability was detected by cell counting kit-8 （CCK-8） after SW480 cells were treated with capsaicin（50，100，200，300，400，500，600，800，1 000 μmol·L-1） for 12，24，and 48 h to select the concentration of capsaicin which can effectively inhibit proliferation. The cell cycle and apoptosis were detected by flow cytometry after SW480 cells were treated with capsaicin （200，400，800 μmol·L-1） for 24 h. The protein expression levels of TRPV1，p53，p-p53，B-cell lymphoma-2（Bcl-2），Bcl-2-associated X protein（Bax），cleaved cysteinyl aspartate-specific protease-3（cleaved Caspase-3），cleaved Caspase-8，and cleaved poly adenosine diphosphate ribose polymerase （PARP） were detected by Western blot after SW480 cells were treated with capsaicin （200，400 μmol·L-1） for 24 h．In addition，the apoptosis was detected after SW480 cells were treated with TRPV1 microRNA（mRNA） and capsaicin（200 μmol·L-1）. Western blot analysis was used to detect the protein expression levels of the above proteins. Result:As compared with the blank group，capsaicin（≥200 μmol·L-1）significantly inhibited the cell viability of SW480 cells（P<0.01） in dose- and time-dependent manners. The cell cycle was arrested in G2/M phase by 200 and 400 μmol·L-1 capsaicin treatment，and arrested in G1 phase by 800 μmol·L-1 capsaicin treatment （P<0.05）. Flow cytometry showed that capsaicin （200， 400， 800 μmol·L-1） significantly promoted apoptosis of SW480 cells simultaneously（P<0.05，P<0.01）. Western blot showed that capsaicin （200，400 μmol·L-1） significantly up-regulated the protein levels of apoptosis-related proteins（p53，p-p53，Bax，cleaved Caspase-3，cleaved Caspase-8，and cleaved PARP） （P<0.05，P<0.01），and significantly down-regulated Bcl-2（P<0.01）. In addition，siRNA-mediated knockdown of TRPV1 significantly attenuated capsaicin-induced apoptosis and the protein levels of apoptosis-related proteins in SW480 cells（P<0.05，P<0.01）. Conclusion:Capsaicin can inhibit cell proliferation，arrest cell cycle，and induce apoptosis of SW480 cells，and the possible mechanism may be related to TRPV1 activation.
