Effect of Daizongfang on Insulin Resistance of 3T3-L1 Adipocytes Induced by Different Methods
10.13422/j.cnki.syfjx.20211602
- VernacularTitle:代综方对不同方式诱导3T3-L1脂肪细胞胰岛素抵抗的改善作用
- Author:
Jing XU
1
;
Xi-ming LIU
2
;
Xiao-yun ZHU
2
;
Wen-xin MA
1
;
Shou-qiang FU
1
;
Li-wei ZHANG
1
Author Information
1. Beijing University of Chinese Medicine，Beijing 100029，China
2. Guang'anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 100053，China
- Publication Type:Research Article
- Keywords:
Daizongfang;
3T3-L1 adipocytes;
insulin resistance;
model
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):1-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Daizongfang （DZF） on insulin resistance （IR） of adipocytes induced by different methods. Method:The cocktail induction method was adopted to induce the differentiation and maturity of 3T3-L1 preadipocytes. An IR model in mature adipocytes was established by the induction of palmitic acid （PA）， high-concentration glucose （HG）， and dexamethasone （DEX）. DZF extracts at different concentrations （2.0， 0.5， 0.1 g·L-1） intervened for 24 hours. A model group， a rosiglitazone （RSG） group， and a blank control group were set up at the same time. The glucose concentration in the culture supernatant was measured by the glucose oxidase-peroxidase （GOD-POD） method. Glucose consumptions under basic conditions （GBasic） and insulin stimulation （GIns） were calculated to evaluate the insulin sensitivity index （ISI）. The mRNA expression of glucose transporter 4 （GLUT4） was detected by the real-time polymerase chain reaction （PCR）. Result:Compared with the model group， the DZF （2.0 g·L-1） showed increased GBasic， GIns， and ISI in three IR models （P<0.05， P<0.01）. In addition， for the PA-induced IR model， GBasic and GIns in the DZF （0.5 g·L-1） group were elevated （P<0.01）， and GBasic， GIns， and ISI in the RSG group increased （P<0.05， P<0.01）. For the HG-induced IR model， GIns and ISI increased in the DZF （0.5 g·L-1） group （P<0.05）， and GBasic， GIns， and ISI were elevated in the RSG group （P<0.01）. For the DEX-induced IR model， GIns and ISI increased in the RSG group （P<0.01）. In the three models， there were differences among groups with different doses. GBasic， GIns， and ISI in the high-dose DZF group increased in varying degrees compared with those in the medium- and low-dose DZF groups （P<0.05）. In the three models， the DZF （2.0 g·L-1） group and the RSG group both increased GLUT4 mRNA expression （P<0.05）. Conclusion:DZF can reduce IR of adipocytes induced by HG， DEX， or PA in a dose-dependent manner and increase glucose uptake in an insulin-independent manner， which may be related to the increase in GLUT4 expression.
