Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway
10.13422/j.cnki.syfjx.20212325
- VernacularTitle:健脾消癌方通过lncRNA HOTAIR/JAK2/STAT3信号通路抑制结肠癌细胞株HCT116转移的机制
- Author:
Jiao JIAO
1
;
Qi TANG
1
;
Yi-lan JIANG
2
;
Dong-fang LI
3
;
Jia-qin LIU
2
;
Guang-sheng HU
1
Author Information
1. Graduate School， Hunan University of Chinese Medicine，Changsha 410006，China
2. Hunan Academy of Traditional Chinese Medicine Affiliated Hospital，Changsha 410208，China
3. Hunan Cancer Hospital， Changsha 410013，China
- Publication Type:Research Article
- Keywords:
Jianpi Xiaoai prescription;
colon cancer;
HCT116 cells;
long non-coding RNA Hox transcript antisense intergenic RNA （lncRNA HOTAIR）;
janus kinase 2 （JAK2）;
signal transducer and activator of transcription 3 （STAT3）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):66-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Jianpi Xiaoai prescription on long non-coding RNA Hox transcript antisense intergenic RNA （lncRNA HOTAIR）/Janus kinase 2 （JAK2） /signal transducer and activator of transcription 3 （STAT3） signaling pathway and to explore the potential mechanism of Jianpi Xiaoai prescription in suppressing the metastasis of colon cancer. Method:The expression of lncRNA HOTAIR in different cells was analyzed. Following the treatment of HCT116 cells with 10%，15%，and 20% Jianpi Xiaoai prescription -containing serum， the invasive ability of Jianpi Xiaoai prescription on HCT116 cells was assessed by transwell assay. The mRNA expression levels of lncRNA HOTAIR，JAK2，and STAT3 were measured by Real-time polymerase chain reaction （Real-time PCR）， and the protein expression levels of JAK2， phosphorylated STAT3 （p-STAT3） and STAT3 by Western blot. Result:The highest expression of lncRNA HOTAIR was detected in HCT116 cells. Compared with the blank group， each Jianpi Xiaoai prescription group exhibited a decreased number of invasive cells （P<0.05， P<0.01）. The relative JAK2 mRNA expression in the middle-dose Jianpi Xiaoai prescription group was down-regulated （P<0.05）， and the relative lncRNA HOTAIR mRNA expression in the middle- and high-dose Jianpi Xiaoai prescription groups and the relative JAK2 mRNA expression in the high-dose Jianpi Xiaoai prescription group were remarkably down-regulated （P<0.01）. Compared with the blank group，the relative p-STAT3 protein expression was down-regulated in the middle-dose Jianpi Xiaoai prescription group （P<0.05）， and the relative JAK2 protein expression in the middle- and high-dose Jianpi Xiaoai prescription groups and the relative p-STAT3 protein expression in the high-dose Jianpi Xiaoai prescription group were remarkably down-regulated （P<0.01）. Conclusion:Jianpi Xiaoai prescription effectively inhibits the metastasis of colon cancer cells， which may be related to the inhibition of lncRNA HOTAIR/JAK2/STAT3 signaling pathway.
